Distance Mapping in Proteins Using Fluorescence Spectroscopy

In these studies, we placed a bimane (probe) and Trp or Tyr (quencher) on opposite ... energy barrier (Ea) to this movement is relatively low (∼1.5â...
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Article pubs.acs.org/biochemistry

Distance Mapping in Proteins Using Fluorescence Spectroscopy: Tyrosine, like Tryptophan, Quenches Bimane Fluorescence in a Distance-Dependent Manner Amber M. Jones Brunette and David L. Farrens* Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239-3098, United States S Supporting Information *

ABSTRACT: Tryptophan-induced quenching of fluorophores (TrIQ) uses intramolecular fluorescence quenching to assess distances in proteins too small (