Article pubs.acs.org/ac
DNA Detection Using Origami Paper Analytical Devices Karen Scida, Bingling Li, Andrew D. Ellington,* and Richard M. Crooks* Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th Street, Stop A5300, Austin, Texas 78712-1224, United States S Supporting Information *
ABSTRACT: We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencherlabeled ssDNA competed for hybridization with a fluorophorelabeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection
