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Food Safety and Toxicology
A Rapid, Onsite, Ultrasensitive Melamine Quantitation Method for Protein Beverages Using Time-Resolved Fluorescence Detection Paper Guanghua Li, Du Wang, Aijun Zhou, Yimin Sun, Qi Zhang, Amnart Poapolathep, Li Zhang, Zhiyong Fan, Zhaowei Zhang, and Peiwu Li J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b01016 • Publication Date (Web): 02 May 2018 Downloaded from http://pubs.acs.org on May 2, 2018
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Journal of Agricultural and Food Chemistry
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A Rapid, Onsite, Ultrasensitive Melamine Quantitation Method for Protein
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Beverages Using Time-Resolved Fluorescence Detection Paper
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Guanghua Li†, Du Wang‡, Aijun Zhou*,†, Yimin Sun†, Qi Zhang‡, Amnart
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Poapolathep§, Li Zhang#, Zhiyong Fan#, Zhaowei Zhang*,‡, and Peiwu Li‡
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†
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430074, P. R. China
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‡
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Wuhan 430062, P. R. China; Key Laboratory of Biology and Genetic Improvement of
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Oil Crops, Ministry of Agriculture; Key Laboratory of Detection for Mycotoxins,
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Ministry of Agriculture; Laboratory of Risk Assessment for Oilseeds Products
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(Wuhan), Ministry of Agriculture; National Reference Laboratory for Biotoxin Test,
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PR China, Wuhan 430062, P.R. China
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§
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Bangkok, 10900, Thailand
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#
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*Corresponding author
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Tel: +86 027-86812943; Fax: +86 027-86812862; E-mail:
[email protected] Wuhan Institute of Technology, School of Materials Science and Engineering, Wuhan
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,
Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University,
Hubei Provincial Institute for Food Supervision, Wuhan 430075, P. R. China
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT: To ensure protein beverage safety and prevent illegal melamine use to
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artificially increase protein content, a rapid, onsite, ultrasensitive detection method for
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melamine must be developed because melamine is detrimental to human health and
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life. Herein, an ultrasensitive time-resolved fluorescence detection paper (TFDP) was
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developed to detect melamine in protein beverages within 15 min using a one-step
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sample preparation. The lower limits of detection were 0.89, 0.94, and 1.05 ng/mL,
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and the linear ranges were 2.67–150, 2.82–150, and 3.15–150 ng/mL (R2>0.982) for
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peanut, walnut, and coconut beverages, respectively. The recovery rates were
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85.86–110.60% with a coefficient of variation 0.982)
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for peanut, walnut, and coconut beverages, respectively. A good recovery of
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85.86–110.60% with a coefficient of variation
