A Yolk-like Multifunctional Platform for Multimodal Imaging and

Jan 12, 2015 - A Yolk-like Multifunctional Platform for Multimodal Imaging and Synergistic Therapy Triggered by a Single Near-Infrared Light. Ruichan ...
0 downloads 8 Views 4MB Size
ARTICLE

A Yolk-like Multifunctional Platform for Multimodal Imaging and Synergistic Therapy Triggered by a Single Near-Infrared Light Ruichan Lv,† Piaoping Yang,*,† Fei He,† Shili Gai,† Chunxia Li,‡ Yunlu Dai,† Guixin Yang,† and Jun Lin*,‡ †

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University, Harbin 150001, P. R. China and ‡State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Harbin 130021, P. R. China

ABSTRACT To integrate photodynamic therapy (PDT) with

photothermal therapy (PTT) and chemotherapy for enhanced antitumor efficiency, we developed a mild and rational route to synthesize novel multifunctional GdOF:Ln@SiO2 (Ln = 10%Yb/1% Er/4%Mn) mesoporous capsules using strong up-conversion luminescent (UCL) GdOF:Ln as cores and mesoporous silica layer as shells, followed by modification with varied functional groups onto the framework. It was found that due to the codoped Yb/Er/Mn in GdOF, the markedly enhanced red emission can efficiently transfer energy to the conjugated PDT agent (ZnPc) which produces high singlet oxygen, and the incorporated carbon dots outside the shell can generate obvious thermal effect under 980 nm laser irradiation and also prevent the premature leaking of ZnPc. Simultaneously, the as-produced thermal effect can obviously enhance the doxorubicin (DOX) release, which greatly improves the chemotherapy, resulting in a synergistic therapeutic effect. The system exhibits drastically enhanced therapeutic efficiency against tumor growth, as demonstrated both in vitro and in vivo. Especially, the doped rare earth ions in the host endow the material with excellent UCL imaging, magnetic resonance imaging (MRI), and computed tomography (CT) imaging properties, thus realizing the target of multimodal imaging guided multiple therapies. KEYWORDS: photodynamic therapy . photothermal therapy . bioimaging . up-conversion . GdOF

I

n recent years, because most of anticancer drugs may have strong toxicity and side-effect with a large dosage, researchers have focused on synthesizing the functional materials with special structure in order to encapsulate diverse functional groups and particles, such as anticancer drugs, photothermal therapy (PTT) and photodynamic therapy (PDT) photosensitizers (PSs) onto the carriers, in order to control the release process or achieve the targeted therapy without damage to normal organs.19 Up to now, a large number of functional structures have been utilized as main drug-carriers in the drug delivery systems (DDSs). Among them, silica-based functional materials have especially been applied as effective carriers for DDSs due to their mesoporous structure,

LV ET AL.

large surface area, tunable pore size, optical transparency, and facile surface modification.1015 In the field of material synthesis, most of the core/shell and hollow structures are spheres which are facile to obtain. However, the elliptical capsules with high length-diameter ratio which may have different endocytosis,16,17 are relatively less reported, especially for yolk-like coreshell structures. To the DDSs, if referred to image-guided PDT and PTT, two separate lights with different wavelengths were often adopted to realize imaging and therapy which makes it difficult for real-time monitoring and efficacy assessment. Therefore, near-infrared (NIR) excitation at 980 nm is potentially used as the up-conversion emissions because UCL emissions in visible regions could be VOL. 9



NO. 2



* Address correspondence to [email protected], [email protected]. Received for review November 7, 2014 and accepted January 12, 2015. Published online January 12, 2015 10.1021/nn5063613 C 2015 American Chemical Society

1630–1647



2015

1630 www.acsnano.org

LV ET AL.

photothermal) agent and drug carriers for multiple anticancer therapy (PDT, PTT, and chemo-). Zinc(II)phthalocyanine (ZnPc) PSs were modified to endow GdOF:Ln@SiO2 with ability to product singlet oxygen (1O2) when excited by red emission derived from NIR irradiation, and the attached carbon dots are to generate thermal effect under NIR laser irradiation. Simultaneously, the produced thermal effect induced enhanced DOX release is to generate a synergistic therapeutic effect. Moreover, the MRI, X-ray CT together with the UCL imaging in vitro and in vivo were employed to investigate the imaging properties. The biocompatibility, the drug loading and release, the cytotoxicity to HeLa cells in vitro, the anti-H22 tumor therapy in vivo and the histologic section analysis were investigated as well.

ARTICLE

utilized as the donors for transferring energy to PDT or PTT agents.1823 Meanwhile, NIR laser located within the optical transmission window of biological specimens has the merits of high detection sensitivity, large penetration depth, increased image contrast, and decreased damage to cells,2429 which are importantly utilized for biological cells and living organisms.3036 Lanthanide oxyfluorides (LnOF) have been considered as excellent luminescent host materials for achieving efficient up-conversion luminescent properties, which are even better than lanthanide fluoride.37,38 In particular, Gd3þ ions can play an effective role as the intermediate sublattice in enhancing the doped activators efficiency, and Gd3þ/Yb3þ doped particles have been proved excellent CT contrast agents due to the high atomic and strong X-ray attenuation.39,40 Meanwhile, Gd-based UCL particles can be used as contrast agents for MRI due to their unpaired 4f electrons.41,42 However, most of the researchers focus on LnOF bulk crystals or regular particles prepared by some limited chemical routes,43,44 and there is no literature on the GdOF particles with functional structure for biomedical application. For a secure and efficient PDT, it requires high 1O2 quantum yield, short irradiation time, and low irradiation pump power (