Here's how you can MEASURE OPTICAL PATH DIFFERENCE with the AO-Baker Interference Microscope
GENERAL ATOMIC Div. of General Dynamics Corp. 1 Rockefeller Plaza New York, N. Y.
Exhibiting: Triga nuclear reactors for neutron activation analysis and radio isotope production, activation analysis service available to industry for ultra sensitive determinations of trace ele ment concentrations, research into and development of new activated analysis application and techniques. GENERAL CHEMICAL DIV. Allied Chemical Corp. 40 Rector St. New York 6, Ν. Υ. Exhibiting: Baker & Adamson labo ratory reagents and fine chemicals for industrial, scientific, and educational use. From among more than 1000 products bearing B&A's "Shield of Quality," its line of electronic chemi cals, radioactive chemicals, and rare earth oxides and salts are featured. GENERAL ELECTRIC CO. X-Ray Dept. 4855 Electric Ave. Milwaukee 1, Wis. Exhibiting: XRD-6 high voltage power supply, SPG No. 3 and SPG No. 6 detectors, constant voltage ca pacitor, spectrometers. GOW-MAC INSTRUMENT CO. 100 Kings Rd. Madison, N. J.
1 B First, as shown in die photomicro graph* above, the microscope ana lyzer was rotated until the background was brought to extinction. Readings were taken directly from the analyzer scale. Averaged settings resulted in read ing of 70.4°.
3
The Optical Path Difference, in degrees, is tw'ia the difference between the two readings:
O P D = 2 (138.2°-70.4°) = 135.6°; or O P D = I
„ „ ) .546 = .206 Microns.
\ 360
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Optical path difference measurements can be made to an optimum accuracy of 1/300 wavelength. This unique ability to measure optical path thick nesses is in itself of great importance. But even more important, these meas urements can be converted into a vari ety of quantitative information of great potential value. Water and protein con tent of a cell, for example, may be meas ured. Materials such as glass, plastics, emulsions, textiles can be examined.
Exhibiting: Detectors for gas chroma tography, gas density and thermal conductivity types; micro cells (capil lary) ; hot wire filaments of tungsten and nickel; new high-resistance fila ments; DC power supplies, tempera ture-controlled detectors. THE EMIL GREINER CO. 2 0 - 2 6 No. Moore St. New York 13, Ν. Υ. Exhibiting: Pipettors, glove box, elec tric resistance thermometer, flow meters, manometers, incubator. W. M. J. HACKER & Co., INC. P.O. Box 6 4 6 West Caldwell, N. J. Exhibiting: Reichert research polariz ing microscope Zetopan-Pol, Reichert Kofler thermo apparatus (microscope hot and cold stages), Reichert appara tus for high temperature microscopy including the Reichert metallograph Universal camera microscope MeF and vacuum heating stage Vacutherm, Struers electrolytic and diamond speci men preparation equipment for metal lography, Linseis dilatometer working on electrical/electronic principles, Linseis thermo-vacuum balance, Linseis differential thermo analysis equipment.
2 . Next, the analyzer was rotated until the nucleus of the cell was brought to extinction. Average settings resulted in reading of 138.2°.
While the AO-Baker Interference Microscope is primarily a quantitative instrument, it also offers unique advantages for qualitative observations through variable intensity contrast and dramatically effective variable color contrast. •Photomicrographs taken by Mr. Lynn C. Wall, Medical Division, Eastman Kodak Co. Data: Epithelial Cell. AO-Baker Interference Microscope, 40X Shearing objective, 10X eyepieces. Corning filter CS4-120 with AO Model 63 0 Pulsarc Illuminator to transmit monochromatic light at .546 microns.
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INFORMATION
Dcpt. B-48. Please Send me your NEW COLOR BROCHURE SB7 on the AO-BAKER INTERFERENCE MICROSCOPE. NAME_ ADDRESS, _ZONE
CITY
STATE_
Circle No. 8 on Readers' Service Card VOL. 34, NO. 2, FEBRUARY 1 962
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