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Animal feces contribute to domestic fecal contamination: Evidence from E. coli measured in water, hands, food, flies and soil in Bangladesh Ayse Ercumen, Amy Janel Pickering, Laura H. Kwong, Benjamin Arnold, Sarker Masud Parvez, Mahfuja Alam, Debashis Sen, Sharmin Islam, Craig Kullmann, Claire Chase, Rokeya Ahmed, Leanne Unicomb, Stephen Luby, and John M. Colford Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b01710 • Publication Date (Web): 07 Jul 2017 Downloaded from http://pubs.acs.org on July 9, 2017
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Environmental Science & Technology
Animal feces contribute to domestic fecal contamination: Evidence from E. coli measured in water, hands, food, flies and soil in Bangladesh Ayse Ercumen1*, Amy J. Pickering2 §, Laura H. Kwong3, Benjamin F. Arnold1, Sarker Masud Parvez4, Mahfuja Alam4, Debashis Sen4, Sharmin Islam4, Craig Kullmann5, Claire Chase5, Rokeya Ahmed6, Leanne Unicomb4, Stephen P. Luby7, John M. Colford Jr.1 1. School of Public Health, University of California, Berkeley, CA, 94720, USA 2. Civil and Environmental Engineering, Tufts University, Medford, 02153, MA, USA 3. Civil and Environmental Engineering, Stanford University, Stanford, CA, 94305, USA 4. Infectious Disease Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, 1212, Bangladesh 5. Water Global Practice, World Bank, Washington, DC, 20433, USA 6. Water Global Practice, World Bank, Dhaka, 1207, Bangladesh 7. Infectious Diseases & Geographic Medicine, Stanford University, Stanford, CA, 94305, USA * Corresponding author § Co-primary author with equal contribution Corresponding author: Ayse Ercumen, PhD Division of Epidemiology, School of Public Health University of California, Berkeley 101 Haviland Hall, MC #7358 Berkeley, CA 94720-7358 (510) 225 8828
[email protected] Running title: Animal feces and domestic fecal contamination Conflicts of interest: None of the authors have any conflicts of interest to declare. Word count for abstract: 206 Word count for text: 5463 Word count for tables/figures: 5 x 300=1500
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Abstract Fecal-oral pathogens are transmitted through complex, environmentally mediated pathways. Sanitation interventions that isolate human feces from the environment may reduce transmission but have shown limited impact on environmental contamination. We conducted a study in rural Bangladesh to (1) quantify domestic fecal contamination in settings with high on-site sanitation coverage; (2) determine how domestic animals affect fecal contamination; and (3) assess how each environmental pathway affects others. We collected water, hand rinse, food, soil and fly samples from 608 households. We analyzed samples with IDEXX Quantitray for the most probable number (MPN) of E. coli. We detected E. coli in source water (25%), stored water (77%), child hands (43%), food (58%), flies (50%), ponds (97%) and soil (95%). Soil had >120,000 mean MPN E. coli per gram. In compounds with vs. without animals, E. coli was higher by 0.54 log10 in soil, 0.40 log10 in stored water and 0.61 log10 in food (p
