Monograph pubs.acs.org/doi/book/10.1021/acsreagents
Anthrone (9(10H)-Anthracenone) Part 4, Monographs for Reagent Chemicals: General Descriptions, Specifications, and Tests eISBN: 9780841230460 Tom Tyner Chair, ACS Committee on Analytical Reagents James Francis Secretary, ACS Committee on Analytical Reagents
ABSTRACT This monograph for Anthrone provides, in addition to common physical constants, a general description including typical appearance, applications, change in state (approximate), and aqueous solubility. The monograph also details the following specifications and corresponding tests for verifying that a substance meets ACS Reagent Grade specifications including: Melting Point, Sensitivity to Carbohydrates, Absorbance of Reagent Solution, and Solubility in Ethyl Acetate.
C14H10O
Formula Wt 194.23
CAS No. 90-44-8
GENERAL DESCRIPTION Typical appearance . . . . . . . . Applications . . . . . . . . . . . . . Change in state (approximate) . Aqueous solubility . . . . . . . . .
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pale yellow solid colorimetric determination of sugar and animal starches melting point, 153–159 °C insoluble
SPECIFICATIONS Melting point . . . . . . . . . . . . . . . . . . . . . . . No more than a 5° range, including 156 °C Sensitivity to carbohydrates. . . . . . . . . . . . . . Passes test Maximum Allowable Absorbance of reagent solution . . . . . . . . . . . Passes test Solubility in ethyl acetate . . . . . . . . . . . . . . . Passes test
© 2017 American Chemical Society
A
DOI:10.1021/acsreagents.4039 ACS Reagent Chemicals, Part 4
ACS Reagent Chemicals
Monograph
pubs.acs.org/doi/book/10.1021/acsreagents
TESTS Melting Point [Part 2: Measurement of Physical Properties; Melting Point].
Sensitivity to Carbohydrates Mark four 25 mL volumetric flasks as 0 µg (blank), 250 µg, 500 µg, and 750 µg. Add the glucose standard (described below) and water as specified in the following table, and dilute to volume with the reagent solution (described below). Place all flasks in a vigorously boiling water bath for 7.0 min exactly. Chill the flasks quickly to room temperature, and determine the absorbance of each at 620 nm against the blank in 1.00 cm cells. A plot of the absorbance against concentration should be linear, and the absorbance of the 750 µg flask should not be less than 0.90.
For the Determination of Sensitivity to Carbohydrates
G l u c o s e S t a n d a r d . (1000 µg in 1 mL). Dissolve 1.00 g of glucose in water, and dilute with water to 100 mL. Pipette 10.0 mL of this solution (1 mL = 10 mg) into a 100 mL volumetric flask, and dilute to volume with water. Re a g e n t S o l u t i o n . Slowly add 132 mL of sulfuric acid to 68 mL of ice-cold water in an ice bath. Allow the mixture to cool to room temperature, add 0.100 g of the sample, and stir if necessary to dissolve.
For the Determination of Sensitivity to Carbohydrates
Flask (µg)
Glucose Standard (mL)
Water (mL)
0
0.00
0.75
250
0.25
0.50
500
0.50
0.25
750
0.75
0.00
Absorbance of Reagent Solution Measure the absorbance of the reagent solution (see the test for sensitivity to carbohydrates) in a spectrophotometer, using 1.00 cm cells, with sulfuric acid as a reference. Record the absorbance at 425 and 620 nm, respectively. The absorbance should not exceed 0.75 at 425 nm and 0.045 at 620 nm.
Solubility in Ethyl Acetate Dissolve 1 g in 50 mL of freshly distilled ethyl acetate in a dry 50 mL glass-stoppered graduated cylinder. The mixture should not be heated, dissolution should be complete, and the resulting solution should be clear.
© 2017 American Chemical Society
B
DOI:10.1021/acsreagents.4039 ACS Reagent Chemicals, Part 4
