Subscriber access provided by - Access paid by the | UCSB Libraries
Characterization of Natural and Affected Environments
Antibiotic Resistome Associated with Smallscale Poultry Production in Rural Ecuador Xueping Guo, Robert D. Stedtfeld, Hayden Hedman, Joseph N.S. Eisenberg, Gabriel Truebac, Daqiang Yin, James M. Tiedje, and Lixin Zhang Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.8b01667 • Publication Date (Web): 26 Jun 2018 Downloaded from http://pubs.acs.org on June 27, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 32
Environmental Science & Technology
1
Antibiotic Resistome Associated with Small-scale Poultry Production in
2
Rural Ecuador
3
Xueping Guo1,2,3, Robert D Stedtfeld4, Hayden Hedman5, Joseph NS Eisenberg6, Gabriel
4
Truebac7, Daqiang Yin1, James M Tiedje2,3.8, Lixin Zhang8,9 *
5
1
6
Science and Engineering, Tongji University, Shanghai 200092, China
7
2
8
Michigan 48824, USA
9
3
Center for Microbial Ecology, East Lansing, Michigan 48824, USA
10
4
Department of Civil and Environmental Engineering, Michigan State University, East
11
Lansing, Michigan 48824, USA
12
5
13
48109, USA
14
6
Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109, USA
15
7
Institute of Microbiology, Colegio de Ciencias Biológicas y Ambientales, Universidad San
16
Francisco de Quito, Quito 170157, Ecuador
17
8
18
Lansing, Michigan 48824, USA
19
9
20
Michigan 48824, USA
21
*Corresponding Author: Lixin Zhang
22
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing,
School for Environment and Sustainability, University of Michigan, Ann Arbor, Michigan
Department of Microbiology and Molecular Genetics, Michigan State University, East
Department of Epidemiology and Biostatistics, Michigan State University, East Lansing,
909 Fee Road, East Lansing, MI 48824 1
ACS Paragon Plus Environment
Environmental Science & Technology
23
Page 2 of 32
[email protected] 2
ACS Paragon Plus Environment
Page 3 of 32
Environmental Science & Technology
24
TOC/ABSTRACT ART
25
26
3
ACS Paragon Plus Environment
Environmental Science & Technology
27
Page 4 of 32
ABSTRACT
28
Small-scale poultry farming is common in rural communities across the developing
29
world. To examine the extent to which small-scale poultry farming serves as a reservoir for
30
resistance determinants, the resistome of fecal samples was compared between production
31
chickens that received antibiotics and free-ranging household chickens that received no
32
antibiotics from a rural village in northern Ecuador. A qPCR array was used to quantify
33
antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) using 248 primer
34
pairs; and the microbiome structure was analyzed via 16S rRNA gene sequencing. A large
35
number of ARGs (148) and MGEs (29) were detected. The ARG richness in production
36
chickens was significantly higher than that of household chickens with an average of 15 more
37
genes detected (p
