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Bioactive Constituents, Metabolites, and Functions
Antioxidant and Anti-Inflammatory Activities of Buffalo Milk #-Valerobetaine Nunzia D'Onofrio, Anna Balestrieri, Gianluca Neglia, Alessandra Monaco, Marco Tatullo, Rosario Casale, Antonio Limone, Maria Luisa Balestrieri, and Giuseppe Campanile J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b07166 • Publication Date (Web): 20 Jan 2019 Downloaded from http://pubs.acs.org on January 20, 2019
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Journal of Agricultural and Food Chemistry
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J Agric Food Chem revision
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Antioxidant and Anti-Inflammatory Activities of Buffalo
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Milk δ-Valerobetaine
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Nunzia D’Onofrio§, Anna Balestrieri‡, Gianluca Neglia#, Alessandra Monaco§, Marco Tatullo†,
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Rosario Casale§, Antonio Limone‡, Maria Luisa Balestrieri§*, Giuseppe Campanile#
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§
Department of Precision Medicine, University of Campania “L. Vanvitelli”, 80138 Naples, Italy.
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Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici (Naples), Italy.
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#
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†
Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy. Tecnologica Research Institute, Biomedical Section, 88900 Crotone, Italy
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*Correspondence: Maria Luisa Balestrieri, Department of Precision Medicine, University of
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Campania “L. Vanvitelli”, via L. De Crecchio 7, 80138, Naples, Italy. Tel.: +39 081 5665865; Fax:
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+39 081 5665863. Email:
[email protected] 18
ORCID: 0000-0001-6001-1789
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Key words: δ-Valerobetaine, Buffalo milk, SIRT1, SIRT6, Hyperglycaemia.
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1 ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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δ-Valerobetaine (δVB), a constitutive metabolite of ruminant milk, is produced in the rumen from
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free dietary Nε-trimethyllysine occurring ubiquitously in vegetable kingdom. Biological role of δVB
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is poorly known. Here, the antioxidant and anti-inflammatory potential of buffalo milk δVB was
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tested in vitro during high-glucose (HG)-induced endothelial damage. Results indicated that δVB
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(0.5 mM) ameliorated the HG cytotoxicity (0.57±0.02 vs. 0.41±0.018 O.D. (P
