Be Among the First to Attend this NEW Hands-On, Lecture-Laboratory Course! The American Chemical Society Presents
Bench Top GC/MS
. 7.10, l " JUW 7 . ; Teen
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If you want to learn how to operate bench top GC/MS systems, but have little experience in this area, this course is for you! Here's what you'll learn: • • • • •
How to set-up, calibrate, and operate MS systems Tips for troubleshooting GC, MS, and data systems How to install, operate, and optimize GC columns How to perform qualitative and quantitative analyses by GC/MS Applications of GC/MS in environmental, pharmaceutical, forensic, and petroleum laboratories
. . . P L U S , YOU'LL GAIN HANDS-ON EXPERIENCE WORKING WITH STATE-OF-THE-ART EQUIPMENT FROM MAJOR MANUFACTURERS
Instructors: Harold M. McNair, Professor and Chairman, Chemistry Department, Virginia Tech Humberto Gomez, Professor, National University
Register today! Enrollment is limited to 20 participants. For more information, mail in this coupon or phone the ACS Continuing Education Short Course Office today at (800) 227-5558 (TOLL FREE) or (202) 872-4508. FAX: (202) 872-6336.
American Chemical Society Dept. of Continuing Education Meeting Code VPI9207 1155 Sixteenth Street, N.W. Washington, DC 20036 YES! Please send me information Bench Top GC/MS (BTOP9207), the NEW ACS Short Course to be offered at Virginia Tech, July 7-10, 1992. Name Title Address City, State, Zip
544 A • ANALYTICAL CHEMISTRY, VOL. 64, NO. 9, MAY 1, 1992
REPORT t h a t a h e t e r o g e n e o u s zone c a n be r e producibly stopped in t h e observat i o n field of a microscope, t h u s allowing fluorescence m e a s u r e m e n t s . T h e i d e a of F I C w a s c o n c e i v e d t h r e e y e a r s ago i n c o o p e r a t i o n w i t h P e t e r R a b i n o v i t c h , a n d t h e concept of F I F M was considered a year later. In t h e y e a r since t h e project s t a r t e d , t h e c o n c e p t s of F I C (32) a n d F I F M (20) have been verified and developed f u r t h e r . A s w o r k c o n t i n u e s , novel a s p e c t s of t h e u s e of F I in cell biology a r e c o m i n g to light. It is h o p e d t h a t this development will parallel t h e previous p a t t e r n in which FI successfully e n h a n c e d m a n y t e c h n i q u e s of i n s t r u m e n t a l a n a l y s i s (33). We express our gratitude to Peter S. Rabinovitch, Department of Pathology, University of Washington, for stimulating discussions on many aspects of flow cytometry and to Ole Thastrup and Rudi Pedersen, ZymoGenetics, Inc., for their assistance and advice on cell biology and video microscopy. Stimulating discussions with Gary D. Christian and Kurt M. Scudder are also much appreciated. This work was supported by NIH (grant no. SSS-3 (5) R01 GM 45260-2).
References (1) The Microbe Hunters; de Kruif, P., Ed.; Harcourt, Brace & World: New York, 1926. (2) Applications of Fluorescence in the Biomedical Sciences; Taylor, D. L.; Waggoner, A. S.; Murphy, R. F.; Lanni, F.; Birge, R. R., Eds.; A. R. Liss: New York, 1986. (3) Flow Cytometry and Sorting, 2nd éd.; Melamed, M. R.; Lindmo, T.; Mendelsohn, M. L., Eds.; Wiley-Liss: New York, 1990. (4) Methods in Cell Biology; Tartakoff, A. M., Ed.; Academic Press: New York, 1989; Vol. 31. (5) Waggoner, A. S. In Applications of Fluorescence in the Biomedical Sciences; Taylor, D. L.; Waggoner, A. S.; Murphy, R. F.; Lanni, F.; Birge, R. R., Eds.; A. R. Liss: New York, 1986; pp. 3 - 2 8 . (6) Muirhead, K ; Horan, P. K.; Poste, G. Bio/Tech. 1985, 3, 337. (7) Arndt-Jovin, D. J.; Jovin, T. M. In Methods in Cell Biology. Fluorescence Microscopy of Living Cells in Culture; Taylor, D. L.; W a n g , Y. L., E d s . ; Academic Press: San Diego, 1989; Vol. 30, P a r t B. (8) Krishan, A. Cytometry 1987, 8, 6 4 2 45. (9) Chused, T. M.; Wilson, Η. Α.; Selimann, B. E.; Tsien, R. In Applications of Fluorescence in the Biomedical Sciences; Taylor, D. L.; Waggoner, A. S.; Murphy, R. F.; Lanni, F.; Birge, R. R., Eds.; A. R. Liss: New York, 1986; pp. 531-44. (10) Rabinovitch, P. S.; June, C. H. In Flow Cytometry and Sorting, 2nd éd.; Melamed, M. R.; Lindmo, T.; Mendelsohn, M. L., Eds.; Wiley-Liss: New York, 1990; pp. 651-68. (11) Grynkiewicz, G.; Poenie, P.; Tsien, R. Y. /. Biol. Chem. 1985, 266X6), 3 4 4 0 50. (12) H a u g l a n d , R. P. Molecular Probes: Handbook of Fluorescent Probes and Research Chemicals; Molecular Probes, Inc.: Eugene, OR, 1989.
