BIOTECHNOLOGY PROGRESS Biotechnology Progress (ISSN 8756-7938) is copublished bimonthly by the American Chemical Society, 1155 16th St., N.W., Washington, DC 20036, and the American Institute of Chemical Engineers, 345 East 47th St., New York, NY 10017. Second-class postage paid at New York, NY, and additional mailing offices. POSTMASTER: Send address changes to Biotechnology Progress, Member & S u b s c r i b e r S e r v i c e s , P . O . Box 3337, Columbus, OH 43210 (614) 447-3776; (800) 333-9511. Canadian GST Reg. No. R127571347. Printed in the USA. Published with the cooperation of the Society for Industrial Microbiology, 3929 Old Lee Highway, Fairfax, VA 22030; (703) 691-3357. MANAGEMENT BOARD American Chemical Society Robert H. Marks Charles R. Bertsch American Institute of Chemical Engineers Stephen R. Smith Mark D. Rosenzweig Journals Department American Chemical Society 2540 Olentangy River Road P.O. Box 3330 Columbus, OH 43210 (614) 447-3600, Ext. 3171 TELEX 6842086 FAX (614) 447-3745 Manager: Mary E. Scanlan Journals Editing Manager: Anne C. O'Melia Assistant Editor: Alena R. Miller-Roush Publications Directorate American Institute of Chemical Engineers 345 East 47th Street New York, NY 10017 (212) 705-7338 FAX (212) 752-3294 Managing Editor: Maura Mullen Advertising Office: Centcom, Ltd., 1599 Post Road East, P.O. Box 231, Westport, CT 06881
© Copyright 1995 by the American Chemical Society and the American Institute of Chemical Engineers. C o p y r i g h t p e r m i s s i o n : Reprographic copying beyond that permitted by Section 107 or 108 of the U.S. Copyright Law is allowed, provided that the appropriate per-copy fee is paid directly to the Copyright Clearance Center, Inc., 222 Rosewood Dr., Danvers, MA 01923. Anyone may excerpt up to 200 words per article for noncommercial, scholarly purposes with appropriate credit to the source. Reprint permission requests: For material published prior to 1990, write to Karen Simpson at AIChE; for material published after J a n u a r y 1, 1990, write to the ACS Copyright Office, Publications Division, 1155 Sixteenth St., N.W., Washington, DC 20036. The paper used in this publication meets the minimum requirements of American National Standard for Information Sciences—Permanence of Paper for Printed Library Materials, ANSI Z39.48-1984. Editorial Information I n s t r u c t i o n s for a u t h o r s are printed in each issue. Please conform to these instructions when submitting manuscripts. M a n u s c r i p t s for p u b l i c a t i o n should be submitted to the Editor, Jerome S. Schultz. Correspondence regarding accepted papers and proofs should be directed to the Journals Department, American Chemical Society, at the address given. Bulk reprints of individual articles are available. For information contact Publication Sales Department, AIChE. Phone: (212) 705-7657. The American Chemical Society, the American Institute of Chemical Engineers, and their Editors assume no responsibility for the statements and opinions advanced by contributors. Registered names and trademarks, etc., used in this publication, even without specific indication thereof, are not to be considered unprotected by law. The document number appears after the bibliographic information for each paper. A document number is a nine character code that is unique to each article and is assigned during the editorial production of the manuscript. It serves as a link between the documents in the printed product and the electronic products (CJACS Plus Images and journals on CD-Rom) and makes it
easy to retrieve the full text of the exact article. 1995 Subscription and Information
Ordering
Members may share/donate their personal subscriptions with/to libraries and the like, but only 5 years after publication.
Printed Members Nonmembers Microfiche Members Nonmembers
U.S.
Canada and Mexico
Europe*
All Other Countries*
$ 32 $365
$ 37 $370
$ 43 $376
$ 47 $380
$ 32 $365
$ 32 $365
$ 36 $369
$ 36 $369
* Air service included.
AIChE member subscriptions should be sent to AIChE, 345 East 47th St., New York, NY 10017. All other new and renewal subscriptions should be sent with payment to American Chemical Society, D e p a r t m e n t L-0011, Columbus, OH 43268-0011. Microforms and back issue orders should be sent to the Microforms & Back Issues Office at the ACS Washington address. Nonmember subscribers in Japan must enter subscription orders with Maruzen C o m p a n y Ltd., 3-10 N i h o n b a s h i 2-chome, Chuo-ku, Tokyo 103, Japan. Tel: (03) 272-7211. Claims for missing numbers will not be allowed if loss was due to failure of notice of change of address to be received in the time specified; if claim is dated (a) North American: more than 90 days beyond issue date, (b) all other foreign: more than 180 days beyond issue date. Claims are handled by Member & Subscriber Services. C l a i m s for Vols. 1 — 5 should be addressed to AIChE. Supplementary material is noted in the table of contents with a • . It is available as photocopy ($12.00 for up to 3 pages and $1.50 per page for additional pages, plus $2.00 for foreign postage) or a 24x microfiche ($12.00, plus $1.00 for foreign postage). Canadian residents should add 7% GST. See supplementary material notice at end of journal article for number of pages. Orders must state whether for photocopy or for microfiche and give complete title of article, names of authors, journal, issue date, and page numbers. Prepayment is required, and prices are subject to change. Order from Microforms & Back Issues Office a t the ACS W a s h i n g t o n a d d r e s s . Supplementary material also appears in the microfilm edition.
BIOTECHNOLOGY PROGRESS®
VOLUME 11, NUMBER 3
May/June 1995
Copyright 1995 by the American Chemical Society and the American Institute of Chemical Engineers
BIPRET 11(3) 235-356 (1995)
ISSN 8756-7938
EDITOR JEROME S. SCHULTZ C e n t e r for Biotechnology a n d Bioengineering University of P i t t s b u r g h P i t t s b u r g h , P e n n s y l v a n i a 15219 Phone: (412) 383-9712 Fax: (412) 383-9710
ASSOCIATE EDITOR, REVIEWS MICHAEL C. FLICKINGER I n s t i t u t e for Advanced Studies in Biological Process Technology University of M i n n e s o t a St. P a u l , M i n n e s o t a 55108 Phone: (612) 624-9706 Fax: (612) 625-1700
REVIEW
235 Biosorption of Heavy Metals B. V o l e s k y * a n d Z. R. H o l a n
ADVISORY BOARD Fredric Bader Auto Immune James Bailey ΕΤΗ, Zurich Kenneth Bischoff University of Delaware Bruce Dale Texas A&M University J o h n Gerlt University of Maryland Juan Hong University of California—Irvine J e n n i f e r C. H u n t e r - C e v e r a Society for I n d u s t r i a l Microbiology Rakesh Jain Harvard Medical School John Jost Genentech, Inc. Alexander Klibanov Massachusetts Institute of Technology Michael Ladisch Purdue University Douglas Lauffenburger University of Illinois, Urbana-Champaign Daryl Lund Rutgers University Janice Phillips Lehigh University Sharon Shoemaker University of California—Davis Michael Shuler Cornell University Gregory Stephanopoulos Massachusetts Institute of Technology JoAnn Stubbe Massachusetts Institute of Technology James Swartz Genentech, Inc. Elizabeth Theil North Carolina State University
K. Venkat Phyton Catalytic, Inc Henry Wang University of Michigan Howard Weetall National Institute of Science and Technology
TOPICAL PAPER 251 Microbial Polysaccharides of Applied Interest: Ongoing Research Activities in Europe Vittorio Crescenzi BIOSEPARATIONS AND DOWNSTREAM PROCESSING 260 Extraction of Charged Fusion Proteins in Reversed Micelles: Comparison between Different Surfactant Systems Craig E. Forney and Charles E. Glatz* 265 Purification by Immobilized Metal Affinity Chromatography of Human Atrial Natriuretic Peptide Expressed in a Novel Thioredoxin Fusion Protein David L. Wilkinson, Nien-Tung Ma, Chris Haught, and Roger G. Harrison* 270 Surfactant-Coated Lipase Suitable for the Enzymatic Resolution of Menthol as a Biocatalyst in Organic Media Noriho Kamiya, Masahiro Goto,* and Fumiyuki Nakashio APPLIED CELLULAR PHYSIOLOGY AND METABOLIC ENGINEERING 276 Investigation of the Ethanol Tolerance of Clostridium thermosaccharolyticum in Continuous Culture Sunitha Baskaran, Hyung-Jun Ahn, and Lee R. Lynd* 282 Specificity of a Lipase in Ester Synthesis: Effect of Alcohol Neena N. Gandhi, Sudhirprakash B. Sawant,* and Jyeshtharaj B. Joshi 288 Fnr, a Global Transcriptional Regulator of Escherichia coli, Activates the Vitreoscilla Hemoglobin (VHb) Promoter and Intracellular VHb Expression Increases Cytochrome d Promoter Activity Philip S. Tsai, Pauli T. Kallio, and James E. Bailey*