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Blackcurrant Anthocyanins Attenuate Weight Gain and Improve Glucose Metabolism in Diet-Induced Obese Mice with Intact, but Not Disrupted, Gut Microbiome Debora Esposito, Thanakorn Damsud, Mickey Wilson, Mary H. Grace, Renee Strauch, Xu Li, Mary Ann Lila, and Slavko Komarnytsky J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.5b00963 • Publication Date (Web): 12 Jun 2015 Downloaded from http://pubs.acs.org on June 17, 2015
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Journal of Agricultural and Food Chemistry
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Blackcurrant Anthocyanins Attenuate Weight Gain and Improve Glucose Metabolism in
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Diet-Induced Obese Mice with Intact, but Not Disrupted, Gut Microbiome
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Running title: Blackcurrant Anthocyanins and Gut Microbiome
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Debora Esposito1,2, Thanakorn Damsud1§, Mickey Wilson1,2, Mary H. Grace1,2, Renee Strauch1,3,
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Xu Li1,3, Mary Ann Lila1,2, Slavko Komarnytsky1,2*
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Plants for Human Health Institute, North Carolina State University, North Carolina Research
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Campus, 600 Laureate Way, Kannapolis, NC 28081
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Dan Allen Drive, Raleigh, NC 27695
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Place, Raleigh, NC 27695
Department of Food, Bioprocessing & Nutrition Sciences, North Carolina State University, 400
Department of Plant and Microbial Biology, North Carolina State University, 100 Derieux
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§
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Rajamangala University of Technology Srivijaya, 109 Thung Song, Nakhon Si Thammarat,
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Thailand 80110
Current address: Program of Thai Traditional Medicine, Faculty of Science and Technology,
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* Corresponding author
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Phone: (704) 250-5459; Fax: (704) 250-5425; Email:
[email protected] 22 23
Disclosure statement: None
1 ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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Blackcurrant (Ribes nigrum L) is a rich source of anthocyanins, however the relationship
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between their apparently limited bioavailability and significant protection against metabolic
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pathologies is poorly understood. In this study, we examined gastrointestinal distribution of
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blackcurrant anthocyanins and their phenolic acid metabolites in lean and diet-induced obese
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mice with healthy and antibiotic-disrupted microbiome. Daily consumption of low or high fat
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diet supplemented with 1% blackcurrant powdered extract (32% anthocyanins) for 8 weeks
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reduced body weight gain and improved glucose metabolism only in mice with the intact gut
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microbiome. Administration of antibiotic cocktail resulted in the 16-25 fold increase (P
