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J. Agric. Food Chem. 2010, 58, 8048–8055 DOI:10.1021/jf101128f
Characterization of Rapeseed (Brassica napus) Oils by Bulk C, O, H, and Fatty Acid C Stable Isotope Analyses EVA KATHARINA RICHTER,† JORGE E. SPANGENBERG,*,‡ MICHAEL KREUZER,† AND FLORIAN LEIBER† †
Institute of Plant, Animal and Agroecosystem Sciences, ETH Zurich, Universitaetstrasse 2, CH-8092 Zurich, Switzerland, and ‡Institute of Mineralogy and Geochemistry, Building Anthropole, University of Lausanne, CH-1015 Lausanne, Switzerland
Rapeseed (Brassica napus) oils differing in cultivar, sites of growth, and harvest year were characterized by fatty acid concentrations and carbon, hydrogen, and oxygen stable isotope analyses of bulk oils (δ13Cbulk, δ2Hbulk, δ18Obulk values) and individual fatty acids (δ13CFA). The δ13Cbulk, δ2Hbulk, and δ18Obulk values were determined by continuous flow combustion and high-temperature conversion elemental analyzer-isotope ratio mass spectrometry (EA/IRMS, TC-EA/IRMS). The δ13CFA values were determined using gas chromatography--combustion-isotope ratio mass spectrometry (GC/C/IRMS). For comparison, other C3 vegetable oils rich in linolenic acid (flax and false flax oils) and rich in linoleic acid (poppy, sunflower, and safflower oils) were submitted to the same chemical and isotopic analyses. The bulk and molecular δ13C values were typical for C3 plants. The δ13C value of palmitic acid (δ13C16:0) and n-3 R-linolenic acid (δ13C18:3n-3) differed (p < 0.001) between rape, flax, and poppy oils. Also within species, significant differences of δ13CFA were observed (p < 0.01). The hydrogen and oxygen isotope compositions of rape oil differed between cultivars (p < 0.05). Major differences in the individual δ13CFA values were found. A plant-specific carbon isotope fractionation occurs during the biosynthesis of the fatty acids and particularly during desaturation of C18 acids in rape and flax. Bulk oil and specific fatty acid stable isotope analysis might be useful in tracing dietary lipids differing in their origin. KEYWORDS: Brassica napus; rapeseed oil; fatty acid composition; carbon stable isotope; oxygen isotope; hydrogen isotope; GC/FID; GC/MS; EA/IRMS; TC-EA/IRMS; GC/C/IRMS
INTRODUCTION
Rape crops, Brassica napus, Brassica rapa, and other Brassica species (Cruciferae, also known as the mustard family), are believed to be among the oldest plants cultivated by man (1). Rapeseed production in India and China started at least 1500 years ago. B. napus probably developed in Europe during the Middle Ages, first in the Mediterranean area, and by the 15th century, rapeseed was being cultivated in the Rhineland (2). It is not clear when rapeseed oil became an important component of the human diet as food oil in addition to its use as fuel for lamp lighting, soap, and candle production (2, 3). The optimal growing temperature of rape is around 20 °C, and the highest seed oil content is attained when seeds mature between 10 and 15 °C (4). Therefore, it has become an important oilseed crop in various countries in cool temperate northern climates where most other oilseed crops do not grow. Rapeseed oil is the third most important vegetable oil after palm oil and soybean oil. Rape is now grown and traded for the production of rapeseed as animal feed, vegetable oil for human consumption, biodiesel, lubricants, and hydraulic liquids. More *Corresponding author (telephone þ41 21 692 4365; fax þ41 21 692 4305; e-mail
[email protected]).
pubs.acs.org/JAFC
Published on Web 06/10/2010
than 30 countries on five continents cultivate several rapeseed species, and the worldwide rapeseed production in the 2008-2009 season was about 21 million metric tons (5). The leading producers are Canada, China, European Union (EU) countries (Denmark, France, Germany, Italy, the United Kingdom, Spain), and India. Winter type B. napus is the main rapeseed crop in most of Europe, and one of the leading oilseed plants grown in Switzerland, with a production of about 45 million kilograms per year (6). The rising rapeseed demand in EU countries for both the feed industry and biodiesel manufacturers, triggered by the temperature drop in European winters, has strongly motivated the improvements in agronomic techniques, processing methods, client-oriented breeding, genetic manipulations, and seed variety production. Breeding developments led to the production of single-low (low in erucic acid, 22:1n-9) and double-low (low in 22:1n-9 in the oil and low in glucosinolates in the meal) rape cultivars (7). In Canada, all double-low rape varieties are known as canola, and in Europe the term rapeseed includes double-low and other quality varieties, such as the high 22:1n-9 acid rapeseed (industrial rapeseed). The main fatty acid composition varies strongly between rape varieties and their genetic modifications, having as end members high-erucic rapeseed oils (45% 22:1) and high-oleic rapeseed oils (80% 18:1). High-erucic rapeseed oils are also rich in eicosenoic
© 2010 American Chemical Society
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Table 1. Fatty Acid Composition of the Studied Vegetable Oil Samples fatty acid contentb (%)
sample CHOIL-342 CHOIL-350 CHOIL-345 CHOIL-352 CHOIL-327 CHOIL-334 CHOIL-353 CHOIL-349 CHOIL-329 CHOIL-332 CHOIL-354 CHOIL-328 CHOIL-356 CHOIL-337 CHOIL-347 CHOIL-348 CHOIL-346 CHOIL-355 CHOIL-326 CHOIL-331 CHOIL-351 CHOIL-116 CHOIL-117 CHOIL-118 CHOIL-121 CHOIL-113 CHOIL-114 CHOIL-115 CHOIL-122 CHOIL-110 CHOIL-111 CHOIL-112 CHOIL-120 CHOIL-119 CHOIL-357
plant species
cultivar
growing sitea
Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Brassica napus Linum usitatissimum Linum usitatissimum Linum usitatissimum Camelina sativa Papaver somniferum Papaver somniferum Papaver somniferum Papaver somniferum Helianthus annuus Carthamus tinctorius
Bioraps Bioraps Cabriolet Cabriolet Expert Expert Expert Oase Oase Oase Oase Robust Robust Remy Remy Remy Remy Remy Viking Viking Viking unknown unknown unknown unknown
district of Aargau (Aargau, CH) district of Aargau (Aargau, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) Reuenthal (Aargau, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) unknown site (DE) Reuenthal (Aargau, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) Reuenthal (Aargau, CH) Reuenthal (Aargau, CH) Reuenthal (Aargau, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) Reuenthal (Aargau, CH) Reckenholz (Z€urich, CH) Feldbach (Z€urich, CH) Albertschwil (St.Gallen, CH) Albertschwil (St. Gallen, CH) Albertschwil (St. Gallen, CH) Neunkirch (Schaffhausen, CH) St. Otmar (St.Gallen, CH) St. Otmar (St. Gallen, CH) St. Otmar (St. Gallen, CH) Neunkirch (Schaffhausen, CH) Albertschwil (St. Gallen, CH) Albertschwil (St. Gallen, CH) Uzwil (St. Gallen, CH) Neunkirch (Schaffhausen, CH) Neunkirch (Schaffhausen, CH) Dettighofen (Thurgau, CH)
year of palmitic harvest (16:0) 2007 2007 2007 2008 2007 2007 2008 2006 2007 2007 2008 2007 2008 2007 2007 2007 2007 2008 2007 2007 2008 2006 2008 2008 2007 2006 2007 2008 2007 2006 2007 2007 2007 2007 2004
4.2 4.2 4.0 4.0 4.7 4.9 4.7 4.2 4.4 4.3 4.3 4.8 5.0 4.7 4.7 4.7 4.9 4.8 4.5 4.6 4.4 4.1 4.1 4.2 4.6 4.7 4.4 4.5 5.4 8.6 8.8 8.8 9.2 5.8 5.6
stearic (18:0)
oleic (18:1n-9)
linoleic (18:2)
linolenic (18:3)
other fatty acidsc
1.8 1.9 1.5 1.5 1.5 1.5 1.9 1.9 1.9 2.2 1.7 1.8 1.5 1.8 1.8 1.8 1.9 1.7 1.5 1.5 1.5 1.8 1.8 1.7 1.8 6.3 6.3 6.1 2.4 2.2 2.1 2.3 1.8 5.0 1.8
62.6 62.8 70.7 70.4 57.7 57.2 58.9 63.3 62.2 62.6 63.5 59.5 59.1 59.7 59.6 59.8 59.5 59.5 57.5 56.8 58.7 62.7 62.8 62.1 60.1 24.4 23.6 21.7 15.6 18.0 17.1 15.0 11.6 17.1 6.5
17.7 17.7 9.0 9.6 21.7 21.7 20.4 17.8 17.9 17.6 16.9 18.5 17.7 18.9 19.4 19.5 19.2 19.1 22.1 22.5 21.0 17.3 17.3 17.2 19.1 14.9 14.8 15.2 16.7 68.4 69.4 71.2 75.0 69.1 83.6
7.7 7.6 9.1 9.0 8.8 8.8 8.8 7.5 8.4 8.1 8.3 9.4 10.1 9.2 9.0 8.8 8.9 9.3 8.9 9.1 9.2 7.5 7.5 8.8 8.8 47.3 48.5 50.2 36.5 0.8 0.7 0.7 0.7 0.1 0.2
5.9, 2.7 (18:1n-7), 1.2 (20:1n-9) 5.9, 2.4 (18:1n-7), 1.2 (20:1n-9) 5.7, 3.0 (18:1n-7), 1.2 (20:1n-9) 5.6, 3.0 (18:1n-7), 1.1 (20:1n-9) 5.6, 3.0 (18:1n-7), 1.1 (20:1n-9) 6.0, 3.2 (18:1n-7), 1.1 (20:1n-9) 5.1, 2.7 (18:1n-7), 1.1 (20:1n-9) 5.3, 2.4 (18:1n-7), 1.1 (20:1n-9) 5.3, 2.7 (18:1n-7), 1.1 (20:1n-9) 5.3, 2.6 (18:1n-7), 1.0 (20:1n-9) 5.5, 2.9 (18:1n-7), 1.0 (20:1n-9) 6.0, 3.2 (18:1n-7), 1.1 (20:1n-9) 5.2, 3.6 (18:1n-7), 1.1 (20:1n-9) 5.6, 3.0 (18:1n-7), 1.1 (20:1n-9) 5.6, 2.9 (18:1n-7), 1.0 (20:1n-9) 5.5, 2.9 (18:1n-7), 1.0 (20:1n-9) 5.5, 2.9 (18:1n-7), 1.0 (20:1n-9) 6.4; 3.6 (18:1n-7), 1.0 (20:1n-9) 5.4, 2.8 (18:1n-7), 1.1 (20:1n-9) 5.5, 2.9 (18:1n-7), 1.1 (20:1n-9) 5.9, 3.0 (18:1n-7), 1.1 (20:1n-9) 6.4, 2.9 (18:1n-7), 1.3 (20:1n-9) 6.3, 2.8 (18:1n-7), 1.3 (20:1n-9) 6.0, 3.0 (18:1n-7), 0.6 (20:1n-9) 5.6, 2.8 (18:1n-7), 1.1 (20:1n-9) 2.5, 0.6 (18:1n-7), 0.2 (22:0) 2.3, 0.5 (18:1n-7), 0.2 (22:0) 2.3; 0.5 (18:1n-7), 0.2 (22:0) 23.5, 13.5 (20:1n-9), 2.5 (22:1n-9) 1.9, 1.1 (18:1n-7), 0.1 (20:0) 1.8, 1.0 (18:1n-7), 0.1 (20:0) 1.9, 1.0 (18:1n-7), 0.1 (20:0) 1.7, 1.1 (18:1n-7), 0.1 (20:0) 2.9, 0.5 (18:1n-7), 0.7 (20:0) 2.3, 0.6 (18:1n-7), 0.3 (22:0)
a CH, Switzerland; DE, Germany. b The relative abundances are given in weight percent. c 18:1n-7, vaccenic acid; 20:0, arachidic acid; 20:1n-9, eicosenoic acid; 22:0, behenic acid; 22:1n-9, erucic acid.
acid (6% 20:1) and contain significant levels of linolenic acid (10% 18:3n-3) and linoleic acid (14% 18:2n-6) (4). The high-oleic rapeseed oils have a lower content of 18:2n-6 (7%) and 18:3n-3 (5%) (4). Edible rapeseed oil is characterized by a relatively low level of saturated acids (6%) and is high in oleic acid (50-65% 18:1) compared to other vegetable oils. The main polyunsaturated fatty acids are linoleic acid (20-30% 18:2n-6) and linolenic acid (6-14% 18:3n-3), occurring in a favorable omega-6 (n-6) to omega-3 (n-3) ratio (4, 7). The most abundant n-3 fatty acid in rape oil is R-linolenic acid (18:3n-3), which has been found to be beneficial in the primary prevention of cardiovascular diseases and is of high importance for brain development (8-10). Rape oil, with an n-6/n-3 ratio of much lower than 4, is considered to be a very healthy edible oil (8, 10, 11). The fatty acid composition of vegetable oils originated from naturally (organic) bred plants varies with environmental conditions, mainly temperature and water availability, which may be related to some extent with the geographical origin (12). To ensure the purity of edible vegetable oils and fats, and to protect high-quality varieties against adulteration and incorrect processing and labeling of the trading goods, the combined fatty acid composition and bulk and individual fatty acids’ carbon isotope composition has been proven to be a powerful tracer of potential frauds in authenticity (13-19). The carbon isotope
composition (reported as δ13C values, where δ value in % = (Rsample - Rstandard)/Rstandard 1000 and R = 13C/12C) of plants and their products is mainly controlled by the isotopic composition of the fixed CO2, the isotope fractionation accompanying the photosynthetic CO2 uptake, and the isotopic composition and amount of the respired CO2 (20). The most important atmospheric CO2-fixing reaction pathways are C3 and C4 (20). C3 plants (plants adapted to temperate ecosystems, including most vegetables, fruits, and temperate grasses) use the Calvin-Benson cycle for CO2 fixation, and the δ13C values fall into the range from -34 to -22%. The C4 plants use the Hatch-Slack cycle and have lower isotopic fractionation compared to the C3 plants. C4 plants are plants adapted to hot, arid environments, comprising most plants in the tropics, including maize, sugar cane, and savanna grasses, and the δ13C values are generally between -16 and -9%. Rape and all of the members of the Cruciferae are C3 plants. Lipids are the major form of carbon storage in seeds of several plant species (21), and carbon discrimination during their synthesis might differ between individual fatty acids (14, 16). Other factors, including plant variety, water availability, cultivation practices, local atmospheric CO2 concentration, temperature, and air humidity, can induce further δ13C variability in the photosynthetic products (22). The variations in the hydrogen and oxygen isotope composition (reported as δ2H and δ18O values,
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where in the calculation of the δ value R = H/ H or O/ O) of vegetable oils and fats depend mainly on the composition of their source (e.g., hydrogen mainly from water, oxygen from fixed CO2), and soil-water-plant physico- and biochemical (e.g., metabolic) processes before and after incorporation in the plant tissues. Therefore, the combination of bulk carbon, oxygen, and hydrogen stable isotope ratios may provide some information on the geographical origin of vegetable oils (13, 23-25). In this study, we investigated the chemical and carbon isotope composition of the fatty acids (δ13CFA) and the bulk carbon, oxygen, and hydrogen (δ13Cbulk, δ2Hbulk, and δ18Obulk) isotopes of rapeseed oils from different rape cultivars, sites of growth in Switzerland and harvest year. This sample set helped to analyze the importance of factors such as cultivar, site location, local climate, and planting time on the fatty acid and stable isotope composition of rapeseed oils. For comparison, other C3 vegetable oils rich in linolenic acid, such as flax (Linum usitatissimum) oil and false flax (Camelina sativa) oil, and rich in linoleic acid, such as poppy (Papaver somniferum), sunflower (Helianthus annuus), and safflower (Carthamus tinctorius), were submitted to the same chemical and isotopic analyses. The results of this study provide insights into plant-specific carbon isotope fractionation during biosynthesis of individual fatty acids (e.g., 18:0, 18:1, 18:2, and 18:3). 2
1
18
16
MATERIALS AND METHODS Samples. Cold-pressed edible seed oils from six different C3 plant species were collected from local organic farmers in northwestern Switzerland and Germany (34 Swiss and 1 German samples). The samples come from six harvesting sites situated within a radius of 60 km with similar geographical and climatic (moderate humid) conditions, including five Swiss sites in the cantons of Zurich (Feldbach and Reckenholz), Aargau (Reuenthal), and St. Gallen (Albertschwil) and one German site (Table 1). Twenty-one rapeseed oils came from the same organic breeding company (P. Kunz S.A., canton Zurich, Switzerland) and were derived from seven different cultivars, including Bioraps (n = 2), Cabriolet (n = 2), Expert (n = 3), Oase (n = 4), Robust (n = 2), Remy (n = 5), and Viking (n=3). The rapeseed oils were compared with other vegetable oils produced at the same sites, including flax (n=3), false flax (n=1), poppy (n = 4), sunflower (n = 1), and safflower (n = 1). All oil samples, except the safflower oil sample from the 2004-2005 harvest season, are from the harvest seasons between 2006 and 2008. The climate was quite similar during the main growing seasons of April-August 2006, 2007, and 2008, with average temperatures of 15.6, 15.5, and 14.8 °C and mean precipitations of 121, 126, and 128 mm, respectively (26). All samples were stored in 10 mL glass vials with PTFE-lined screw caps at 4 °C in the dark prior to analysis. Sample Preparation. All samples were saponified with methanolic sodium hydroxide prior to conversion of fatty acid to fatty acid methyl ester (FAME) with methanolic BF3. After boiling of approximately 80 mg of pure fat with 2 mL of NaOH (0.5 M) for 3 min, 3 mL of methanolic boron trifluoride (1.3 M) was added and the mixture was heated again for 4 min. The reaction was stopped by adding 7 mL of NaCl (0.34 M) and 4 mL of hexane. The tubes were shaken for 30 s and centrifuged at 4000 rpm before the upper layer was transferred to 2 mL vials for gas chromatographic analysis. Fatty Acid Analysis by GC/FID and GC/MS. The fatty acid composition was determined in duplicate by separating the FAME on an Agilent model 6890 gas chromatograph (GC; Wilmington, DE) equipped with a 30 m 320 μm, 0.25 μm, Supelcowax-10 fused silica column (Sigma Aldrich, Bellefonte, PA) and a flame ionization detector (GC/FID). Hydrogen was used as carrier gas with a flow of 2.2 mL/min. The sample was injected at a temperature of 240 °C and a split of 30:1. The oven temperature program was 1 min at 160 °C, raised at 20 °C/min to 190 °C, raised at 7 °C/min to 230 °C, held for 5 min at 230 °C, and finally raised at 20 °C/min to 250 °C for 12 min; the total time was 26 min. The identification of individual FAME was performed by comparison of retention times with those of a standard FAME mixture (Supelco,
Bellefonte, PA), after characterization of the single fatty acids by gas chromatography-mass spectrometry (GC/MS, Thermo Fisher, Argenteuil, France) equipped with a Supelcowax-10 fused silica column. Chromatograms were evaluated by using HP ChemStation software (HewlettPackard, Palo Alto, CA). The proportion of the different FAME was calculated using the ratio of the peak area of the respective FAME to the sum of total FAME peak areas. C, O, and H Isotope Analyses of Bulk Oils by EA/IRMS and TCEA/IRMS. All stable isotope analyses were performed in the laboratories of the Institute of Mineralogy and Geochemistry at the University of Lausanne. The carbon isotope ratio (13C/12C) was determined by flash combustion on a Carlo Erba 1108 (Milan, Italy) elemental analyzer (EA) connected to a Thermo Fisher Delta V (Bremen, Germany) isotope ratio mass spectrometer (IRMS) that was operated in the continuous helium flow mode via a Conflo II split interface (EA/IRMS). An aliquot of the oil sample (100-500 μg) was wrapped in a tin capsule and combusted in the EA under a stream of helium and oxygen by flash combustion in a quartz reactor at 1020 °C packed with Cr2O3 and (Co3O4)Ag to form CO2, N2, NOx, and H2O. The gases were then passed through a reduction reactor containing elemental copper and copper oxide at 640 °C. Water was subsequently removed by anhydrous Mg(ClO4)2, CO2 was separated in a gas chromatograph with a 5 m 1/4 in. i.d., Pora-PLOT Q packed column (Varian, Palo Alto, CA) at 70 °C and analyzed for its isotopic composition on the IRMS. Reference CO2 gas was inserted in the He carrier flow as pulses of pure standard gas. The oxygen and hydrogen isotope ratios (18O/16O, 2H/H) were measured with a Thermo Fisher high-temperature conversion elemental analyzer (TC-EA) coupled to a Delta Plus XL IRMS via a Conflo II split interface (TC-EA/IRMS). For O and H isotope analyses, separate pyrolysis of aliquots of the oil samples was done in Ag capsules and in a reactor containing a glassy carbon tube filled with glassy carbon grains at 1250 °C. The produced CO and H2 gases were separated in a GC and analyzed in the IRMS. Reference CO and H2 gases were inserted in the He carrier flow as pulses of pure standard gases. The stable isotope composition of carbon, oxygen, and hydrogen are reported in delta (δ) notation as the per mil (%) deviations of the isotope ratio relative to known standards: δ ¼ ½ðRsample - Rstandard Þ=Rstandard 1000 R is the ratio of the heavy to light isotopes (i.e., 13C/12C, 18O/16O, 2H/H). For carbon the standard is Vienna Pee Dee Belemnite limestone (VPDB), and for oxygen and hydrogen it is Vienna Standard Mean Ocean Water (VSMOW). Each analytical sequence consisted of two sets of calibration standards, to test the precision and accuracy of the unknown samples. The repeatability and intermediate precision of the EA/IRMS and TC-EA/ IRMS method, defined as the observed variability from separately replicate analyses of laboratory standard materials (glycine, δ13C = -26.1%; urea, δ13C = -43.1%) (27) and vegetable oil samples, were better than 0.1% (1 SD) for δ13C and 0.3% for both δ18O and δ2H values. The accuracy of the analyses was checked periodically by analyses of the international reference materials USGS-24 graphite (-15.9% δ13C), IAEA-PEF1 polyethylene foil (-31.8% δ13C), and NBS-22 oil (-29.7% δ13C). The δ2H analyses were calibrated with the international standards IAEA-PEF1 (δ2H = -100.3), NBS-22 oil (δ2H = -120.0), IAEACH3 (δ2H = -43.5), and IAEA-CH6 (δ2H = -11.7). The calibration standards used for oxygen were IAEA-601 (δ18O = 23.3), IAEA-602 (δ18O = 71.4), IAEA-CH3 (δ18O = 32.6), IAEA-CH6 (δ18O = 36.4), and an in-house standard UNIL-TP5 (δ18O = 29.8) as described by Spangenberg (27). The accuracy of the isotopic analyses was checked sporadically by analyses of international standards. Isotopic Analysis of Individual Fatty Acids by GC/C/IRMS. The compound-specific stable carbon isotope analyses (δ13C values) of the fatty acids were obtained by the use of an Agilent 6890 GC coupled to a Thermo Fischer Delta V IRMS by a combustion (C) interface III (GC/C/ IRMS) under a continuous helium flow. The combustion interface consists of two ceramic furnaces: an oxidation reactor with CuO/NiO/Pt wires at 940 °C and a reduction reactor with Cu wires at 600 °C. Water was removed from the effluent gas by passing a Nafion tube (Perma Pure, Toms River, NJ) with an annular back-flow of He. The GC was operated with the same type of column (Supelco-Wax 10 column) and temperature program used for GC/FID analyses. The background subtraction and
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Figure 1. GC/FID chromatograms of the fatty acid methyl esters of rape (A), flax (B), false flax (C), poppy (D), sunflower (E), and safflower (F) oils. δ13C values were calculated using ISODAT 7.2 software. The repeatability and intermediate precision of the GC/C/IRMS procedure and the performance of the GC and combustion interface were evaluated by injection of an in-house mixture of n-alkanoic acids (UNIL-FAME-MIX), of known isotopic composition, and at least three replicate analyses of the oil samples. The standard deviations for repeatability ranged between 0.05 and 0.4% for the main FAME and for intermediate precision between 0.3 and 1.1%. The accuracy of the GC/C/IRMS analyses was checked every 10 analyses by injection of a 20:0 methyl ester isotope standard prepared by A. Schimmelman from the Biogeochemical Laboratories at Indiana University, Bloomington, IN. The isotopic shift due to the carbon introduced in the fatty acid methylation was corrected by the mass balance equation (16) δ13 C FAME ¼ f FA δ13 C FA þ f MeOH δ13 C MeOH where δ13CFAME, δ13CFA, and δ13CMeOH are the carbon isotope compositions of the fatty acid methyl ester, the fatty acid, and the methanol used for methylation of the fatty acid, respectively, and fFA and fMeOH are the carbon fractions in the fatty acid methyl ester due to the alkanoic chain and methanol, respectively. The variability introduced by this correction was determined by GC/C/RMS measurements of replicate derivatized aliquots of palmitic (16:0) and stearic (18:0) acids of known isotopic composition (28). The differences of the measured and calculated δ13CFA values are much smaller than the standard deviation for repeatability of GC/C/RMS analyses of FAME from similar C-chain length (28). Statistical Evaluation. Statistical analysis was performed using SAS software (version 9.1, SAS Institute Inc., Cary, NC). Average isotopic values of bulk oils and individual fatty acids were subjected to analysis of variance using the general linear model (GLM procedure), considering separately the different plant species and rape cultivars. Multiple comparisons among means were performed with Tukey’s method. Principal component analysis (PCA) was performed using SPSS software (version 17.0, 2008, SPSS Inc., Chicago, IL) to cluster the isotopic measurements within a limited number of independent variables (principal components).
RESULTS AND DISCUSSION
Fatty Acid Contents. The fatty acid composition of the oil samples differed as expected for the different vegetable oils (Table 1; Figure 1). The concentrations of saturated fatty acids (mainly 16:0) were low in all samples, but important differences in mono- and polyunsaturated fatty acid contents were observed. The highest relative concentrations in oleic acid were observed in rapeseed oils (61.2 ( 3.5%). Flax and false flax oils differed from the other by their high 18:3 content (48.7 ( 1.5 and 36.5%, respectively), whereas poppy, sunflower, and safflower oils were rich in 18:2 (71.0 ( 2.9, 69.1, and 83.6%, respectively), but poor in 18:3. Relatively high 18:3 concentrations were also found in rape oil (8.7 ( 0.7%). Differences in the concentrations of mono- and polyunsaturated fatty acids in the oil samples from different rape cultivars were observed. The lowest concentration of polyunsaturated fatty acids was found for Cabriolet rape (18.4%) and the highest for Viking rape (30.9%). Stable Isotope Composition of Bulk Oils. The δ13C values of all the analyzed bulk oils (-30.8 to -27.6%) were typical for oils from C3 plants (14-19). The differences between the average δ13C values of rape, poppy, and flax oils were small but still significant (p