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Ant-cGMP does not activate PDE5 through binding to the allosteric site, because it ... by the absorbance at 280 nm (AC5100L UV monitor, Atto Co., Ltd.) showed two peaks .... All numerical calculation was done using Microsoft Excel software.
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Binding of cGMP to the GAF domain of PDE5 activates the enzyme, and generates a form that can be phosphorylated by cGMP-dependent protein kinase on ...
The preparation contained PDE5 as the major cGMP-PDE activity and was ... Long-term oral phosphodiesterase 5 inhibitor therapy alleviates recurrent priapism.
Jan 20, 1989 - This threonine is invariant in all cGMP binding domains, but the ... out of 24 cAMP binding sites of protein kinases is alanine, which cannot form ...
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