Capillary electrophoresis with laser-induced fluorescence detection of

Anal. Chem. 1002, 64, 1737-1740

1737

TECHNICAL NOTES

Capillary Electrophoresis with Laser-Induced Fluorescence Detection of PCR Fragments Using Thiazole Orange Herbert E. Schwartz'J and Kathi J. Ulfelder Beckman Instruments, Fullerton, California 92634

INTRODUCTION Capillary electrophoresis (CE) is increasingly used for separation of DNA.'+ Polymer networks, such as those based on linear polyacrylamide and cellulose derivatives have been effective as sieving media in capillaries for the separation of relatively small DNA fragments (i.e.