96
Anal. Chem. 1982, 54, 96-101
Technologies," Gatlinburg, TN, Sept 25-28, 1978, CONF-780903, Oak Ridge National Laboratory: Oak Ridge, TN, 1979; p 153. (201 Natusch. D. F. S.: Tomklns. B. A. Anal. Chem. 1978. 50. 1429-1434. i21) Ho, C.-h.; Guerln, M. R.; Clark, B. R.; Rao, T. K.; Epier, J L J Anal. Toxicol. 1081. 5. 143-147. (22) Heftmann, E., Ed. "Chromatography", 3rd ed.; Van Nostrand-Reinhoid: New York, 1975; p 56. (23) Vogel, A. I. "Practical Organic Chemistry", 3rd ed.; Wiley: New York, 1956; pp 798-799, 1074-1076.
(24) Buchanan, M. V. Oak Ridge National Laboratory, personal communlcation, 1980.
RECEIVED for review April 16, 1981. Accepted October 13, 1981, This work was funded by the U.S, Department of Office of Health and Environmental ReSHU'ch under Contract No. W-7405-eng-26 with the Union Carbide Corp.
Collision-Induced Dissociation with Fourier Transform Mass Spectrometry R. B. Cody, R. C. Burnier, and B. S. Frelser" Department of C h e m M y , Purdue University, West Lafayette, Indiana 47907
Colllslon-induced dissociation is demonstrated on a number of primary and secondary Ions uslng a Nicolet prototype Fourler transform mass spectrometer. Like the triple quadrupole technlque, collision-Induced dissociation uslng FT-MS Is a relatively low energy and efflclent process. The ablllty to study a wlde range of Ion-molecule reaction products is exemplified by results on proton-bound dimers and transltlon metal containing ionic specles. Variation of colllslon energy by varying the RF Irradiation level can provlde information about product distributions as a functlon of energy as well as yleld ion structural Informatlon. Like the triple quadrupole technlque, no slits are employed and vlrtually all of the fragment ions formed by the C I D process may be detected. Unlike all previous mass spectrometric techniques for studying CID, a tandem Instrument Is not required, and dlfferent experiments are performed by making software modifications rather than hardware modifications.
Collision-induced dissociation (CID) remains one of the most useful and widely employed mass spectrometric techniques for ion structure determination and forms the basis for the powerful MS/MS technique for complex mixture analysis. In general, the CID technique consists of accelerating a given ion into a collision gas thereby imparting energy to the ion and inducing fragmentation. The ionic fragments are then mass analyzed, yielding essentially a "mass spectrum" of the precursor ion. While high kinetic energies (3-30 keV) are required to observe CID using mass-analyzed ion kinetic energy spectrometry (MIKES) in reverse-geometry mass spectrometers (1-3),Yost and Enke using a triple quadrupole mass spectrometer recently demonstrated the unexpected result that a low-energy (
