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Journal of Proteome Research
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Journal of Proteome Research
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Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics
Abdelfattah El Ouaamari1§, Jian-Ying Zhou2§, Chong Wee Liew3§, Jun Shirakawa1¥, Ercument Dirice1¥, Nicholas Gedeon1, Sevim Kahraman1, Dario F. De Jesus1, Shweta Bhatt1, Jong-Seo Kim2, Therese RW Clauss2, David G. Camp II2, Richard D. Smith2, Wei-Jun Qian2* and Rohit N. Kulkarni1*
1
Islet Cell & Regenerative Biology, Joslin Diabetes Center, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02215 2
Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific
Northwest National Laboratory, Richland, Washington 99352 3
Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.
Running title: Proteomic Islet Response to Insulin Resistance Keywords: insulin resistance, pancreatic islets, proteome, proliferation, metabolism, function. § ¥
These authors contributed equally to this work These authors contributed equally to this work
*Correspondence to:
Rohit N. Kulkarni:
[email protected] and Tel: (617) 309-3460 Wei-Jun Qian:
[email protected] and Tel: (509) 371-6572 1 ACS Paragon Plus Environment
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Journal of Proteome Research
ABSTRACT Compensatory islet response is a distinct feature of the pre-diabetic insulin resistant state in humans and rodents. To identify alterations in the islet proteome that characterize the adaptive response, we analyzed islets from five-month-old male control, high-fat diet fed (HFD) or obese ob/ob mice by LC-MS(/MS) and quantified ~1,100 islet proteins (at least two peptides) with a false discovery rate
