Competition between Bending and Internal Pressure Governs the

Mar 8, 2017 - *E-mail: [email protected]., *E-mail: [email protected]. ACS AuthorChoice - This is an ..... To solve for the vesicle shape, we minimize t...
0 downloads 13 Views 2MB Size
This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

Competition between Bending and Internal Pressure Governs the Mechanics of Fluid Nanovesicles Daan Vorselen,†,‡ Fred C. MacKintosh,†,§,∥ Wouter H. Roos,*,†,⊥,# and Gijs J.L. Wuite*,†,# †

Department of Physics and Astronomy and LaserLab, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands Department of Oral Function and Restorative Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Research Institute MOVE, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, 1081 LA, The Netherlands § Departments of Chemical & Biomolecular Engineering, Chemistry, and Physics & Astronomy, Rice University, Houston, Texas 77005, United States ∥ Center for Theoretical Biophysics, Rice University, Houston, Texas 77030, United States ⊥ Moleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands ‡

S Supporting Information *

ABSTRACT: Nanovesicles (∼100 nm) are ubiquitous in cell biology and an important vector for drug delivery. Mechanical properties of vesicles are known to influence cellular uptake, but the mechanism by which deformation dynamics affect internalization is poorly understood. This is partly due to the fact that experimental studies of the mechanics of such vesicles remain challenging, particularly at the nanometer scale where appropriate theoretical models have also been lacking. Here, we probe the mechanical properties of nanoscale liposomes using atomic force microscopy (AFM) indentation. The mechanical response of the nanovesicles shows initial linear behavior and subsequent flattening corresponding to inward tether formation. We derive a quantitative model, including the competing effects of internal pressure and membrane bending, that corresponds well to these experimental observations. Our results are consistent with a bending modulus of the lipid bilayer of ∼14kbT. Surprisingly, we find that vesicle stiffness is pressure dominated for adherent vesicles under physiological conditions. Our experimental method and quantitative theory represents a robust approach to study the mechanics of nanoscale vesicles, which are abundant in biology, as well as being of interest for the rational design of liposomal vectors for drug delivery. KEYWORDS: atomic force microscopy (AFM), nanoindentation, SUVs, nanovesicles, membrane mechanics, liposome (GUVs: ∼10 μm). The techniques used for studying GUVs, e.g., micropipette aspiration and optical imaging of shape fluctuations,16,17 are developed for these large vesicles and are less suitable for SUVs.16,17 Instead, for mechanical studies of small vesicles, nanoscale indentations using atomic force microscopy (AFM) have been employed.18−22 However, from these experiments no consistent picture has emerged regarding the underlying mechanical properties. This is partly due to the fact that these nanoindentation studies of SUVs, in contrast to studies of GUVs,16,17 have generally been interpreted using elasticity models with finite shear moduli, which are inappropriate for fluid bilayers that lack a shear modulus. Moreover, the potential influence of pressure has not been considered.

mall unilamellar vesicles (SUVs: ∼0.1 μm) perform multiple vital roles in biology. Prime examples of SUVs in cell biology include synaptic vesicles,1 viral envelopes,2 and extracellular vesicles for cell-to-cell communication.3 In addition, synthetic liposomes of this size are currently used as nanocarriers for drug delivery and developments for further applications continue.4,5 Mechanical properties of natural and synthetic vesicles and nanoparticles are reported to influence their uptake by cells,6−11 a phenomenon that is also supported by theoretical models.12,13 Moreover, the mechanical stability of vesicles is a key limitation of their application for drug delivery.4 Consequently, multiple approaches have been developed to stabilize them.14,15 Therefore, understanding the underlying mechanics of such vesicles is crucial for both understanding biological function and developing effective drug delivery strategies. Although SUVs are an important class of vesicles, measurement of their mechanical properties is still challenging. The vast majority of previous studies of the mechanical properties of vesicles have been performed on giant unilamellar vesicles

S

© 2017 American Chemical Society

Received: October 29, 2016 Accepted: March 8, 2017 Published: March 8, 2017 2628

DOI: 10.1021/acsnano.6b07302 ACS Nano 2017, 11, 2628−2636

Article

www.acsnano.org

Article

ACS Nano

Figure 1. Vesicle size and shape. (a) Topographic images of 200 nm extruded vesicles imaged at ∼100 pN. Dashed lines correspond to the line profiles in b with the same colors. (b) Line profiles through the maximum of 2 vesicles along the slow scanning axes, fitted circular arcs and estimated vesicle shapes after tip deconvolution under assumption that vesicles form spherical caps. (c) Height, fwhm, and radius of curvature determined from images of 100 nm extruded vesicles imaged at various forces. Error bars representing the s.e.m. are present, but are mostly smaller than the marker. For each imaging force >180 particles were analyzed. Linear fits with slope −0.020 (−0.003 to −0.036) (height), −0.056 (−0.069 to −0.045) (fwhm), and −0.053 (−0.063 to −0.042) (Rc) show that apparent fwhm and Rc decrease faster than the height with increasing imaging force (ranges mark 68% confidence intervals). (d) Shape of 200 nm extruded vesicles as characterized by height/Rc (N = 46). Inset shows example shapes corresponding to various ratios. (e) Spherical radius of the vesicles determined by AFM (histogram and Gaussian fits). R0 = 87 ± 14 nm (standard deviation (st.d.), N = 46), R0 = 59 ± 16 nm (st.d., N = 87), and R0 = 31 ± 11 nm (st.d., N = 21) for extruded 200 nm, extruded 100 nm and sonicated vesicles, respectively. Square markers and error bars correspond to vesicle radii obtained with DLS: R0 = 94 ± 20 nm (st.d.), R0 = 75 ± 25 nm (st.d.), and R0 = 39 ± 4 nm (st.d.).

cap,23 allowing determination of the radius of curvature of vesicles (Rc) by subtracting the tip radius (Figure S1). However, soft samples, such as these vesicles, can be affected by forces applied during AFM imaging. To determine the extent of this effect we imaged many 100 nm extruded vesicles at various force set points and noticed that their apparent height and especially width were underestimated already at low imaging forces (Figure 1c). To avoid underestimation of the height of vesicles we can use the zero force contact point from indentations (HFDC), which shows that vesicles are 11 ± 1 nm (standard error of the mean (s.e.m.), number of measurements, each of which obtained on a separate vesicle (N) = 46) higher than the apparent height obtained from images for 200 nm vesicles (Figure S1). We used a subsequent correction for the radius of curvature, which is based on geometric arguments (Figure S1) and the experimental data in Figure 1c, to obtain the vesicle geometry and size. This analysis showed that the adhered liposomes adopt approximately hemispherical shapes (HFDC/Rc ≈ 1) (Figure 1d). Furthermore, these measurements allow calculation of the original vesicle radius before adhesion (R0), assuming surface area conservation (Figure 1e). We repeated these measurements for 100 nm extruded vesicles and sonicated vesicles, showing that the obtained size distributions correspond well with size distributions acquired with dynamic light scattering (DLS). Nanoindentations Reveal Strong Tip Size Dependent Behavior. Next, starting with 200 nm extruded vesicles, we

Here, we present an AFM-based approach to quantify the mechanical properties of small fluid vesicles as well as a model that captures their mechanical response. We performed imaging and nanoindentation measurements on single SUVs of 30−100 nm radius. For accurate measurements of vesicle size and shape we introduced corrections for tip dilation and deformation caused by imaging forces. The mechanical properties were investigated by performing nanoindentations with various AFM tip sizes. In parallel, we developed a model to describe nanoindentation of vesicles, which takes the fluidity of the membrane into account. We then quantitatively compared various aspects of the model with the experimental data, ultimately allowing us to estimate the contributions of bending and pressure to the vesicle stiffness. With the combination of AFM experiments and development of a theoretical model we both deepen the understanding of the mechanics of SUVs and we lay out a framework for more accurate measurements of mechanical properties of SUVs.

RESULTS Size and Shape Measurement of Nanovesicles. First, we imaged vesicles to determine their geometry (Figure 1a). Vesicles of complex lipid mixture, obtained by extrusion through 200 nm filters, were attached to a 0.001% poly Llysine coated surface in PBS. Upon adhesion, we observed spreading of the initially spherical vesicles (Figure 1b). The expected resultant shape of an adherent vesicle is a spherical 2629

DOI: 10.1021/acsnano.6b07302 ACS Nano 2017, 11, 2628−2636

Article

ACS Nano

Figure 2. Force indentation behavior of vesicles. (a) Typical indentation curves obtained on an extruded 200 nm vesicle with a sharp tip (Rt ≈ 18 nm). Various colors represent subsequent indentations. Upper right panel shows the FDC made with the lowest set point, highlighting the overlap between approach (black) and retract (gray). Lower right panel shows a zoom on the dashed box in the main panel, highlighting the bilayer penetration events in the blue curve. (b) FDC made with a 43 nm tip. FDC shows a strong nonlinear response and subsequent discontinuity (Figure S4). Insets show images of a sharp tip (Rt ≈ 18 nm) and a blunt tip (Rt ≈ 43 nm) reconstructed using blind tip reconstruction. Black arrows indicate 50 nm in x,y, and z direction. (c) Average FDCs, constructed from a single FDC per vesicle (each normalized to vesicle radius), for all sharp tips combined, and for individual blunt tips. Legend states tip radius and number of vesicles measured for each condition. Error bars represent 68% confidence intervals of the estimated mean determined by bootstrapping (1000 repetitions). (d) Same data as c, but plotted on logarithmic scale. Force curves show an initial linear regime and subsequent onset of superlinear behavior. Inset shows individual FDCs made with the 43 nm tip.

used to indent multiple vesicles (Figure 2a,b) and create average FDCs using a single FDC per vesicle (Figure S6). When we used the larger tips, we noticed a strong superlinear response (Figure 2b,c). The initial part of the average FDCs made with the various tips overlaps, but larger tips result in an early (0.05−0.1 Rc) stiffening. The initial response for larger tips is approximately linear and the stiffening leads to an exponent of ∼2, which is also observed in individual FDCs (Figure 2d). Interestingly, previous observations of linear behavior were made with smaller tips (Rt ≈ 15 nm)19 than observations of superlinear behavior (Rt ≈ 30 nm)18,20 and with our current results we have a clear explanation for these differences. High Vesicle Stiffness Is Inconsistent with Bending Alone. A single FDC per vesicle from the data gathered with sharp tips was used to measure the effective stiffness K of vesicles in the regime of linear response (0.02−0.1 Rc), resulting in a value of 0.015 ± 0.001 N/m (s.e.m., N = 46) for 200 nm extruded vesicles (Figure S7). Measurements with extruded 100 nm vesicles (K = 0.021 ± 0.001 N/m (s.e.m., N = 84)) and sonicated vesicles (K = 0.032 ± 0.002 N/m (s.e.m., N = 42)) had similar stiffness. To gain insight in the factors contributing to the vesicle stiffness, we proceeded to describe the mechanical behavior in terms of intrinsic membrane properties, i.e., a bending modulus κ and stretch modulus σ.16,17,26,27 Since the applied force is perpendicular to the bilayer plane, the contribution of stretching is expected to be negligible. In case of bending energy alone, the vesicle effective stiffness, with units of energy per length squared, should be of

performed nanoindentations by moving the AFM tip to the center of a vesicle and indenting it multiple times using a preset force, creating force distance curves (FDCs).24 A typical FDC is shown in Figure 2a. Before such an indentation we always checked that we were working with a clean tip (Figure S2). As previously observed,18,19 vesicles can withstand large deformations without permanent damage. This robustness is inferred from the lack of change in contact point after multiple indentations (Figure 2a) and confirmed by imaging afterward (Figure S3). Typically, we first performed a small indentation until 500 pN. The overlap between indentation and retraction suggests that the initial behavior is fully elastic (Figure 2a). In subsequent indentations, we deformed the vesicle until a sudden increase in stiffness (at ∼65 nm indentation in Figure 2a), after which we observed two discontinuities, likely corresponding to the two lipid bilayers being pushed together and penetrated (Figure 2a). The occurrence of only two bilayer penetrations suggests that the vesicles are unilamellar (see Figure S4). Previously, both linear and strong superlinear force−distance relationships were reported in vesicle indentation studies.18−20,22 We reasoned that the origin of this difference could be caused by differences in AFM tip size. To test this hypothesis, we used an approach based on AFM tip wear on high roughness surfaces.25 Such wear leads to increased tip size, while the tip maintains its spherical apex, identical tip material and cantilever properties (Figure 2b, insets and Figure S5). The tip radius (Rt) was estimated using blind tip reconstruction. Next, tips with different radii (Rt = 18, 29, and 43 nm) were 2630

DOI: 10.1021/acsnano.6b07302 ACS Nano 2017, 11, 2628−2636

Article

ACS Nano order κ/R2, where κ is the membrane bending modulus (typically 10−50 kbT for a fluid bilayer).28,29 For vesicles much larger than the membrane thickness, the relevant length scale R should be the vesicle radius of curvature Rc. For the typical radii in our experiments (Rc ∼ 100 nm), the stiffness is expected to be of order ∼10−5 N/m. This strongly suggests that bilayer bending alone cannot account for the 3-orders of magnitude higher stiffness observed experimentally. Therefore, the obtained stiffness is likely dominated by an osmotic pressure difference over the membrane (ΔΠ). Vesicles adhered to the surface are deformed and the lipid bilayer is only able to stretch a few percent.30 Hence, the internal volume of vesicles shrinks and the concentration of membrane impermeable solutes in the lumen goes up, causing an osmotic pressure difference over the membrane. This osmotic pressure in turn will make the vesicle resist indentation and thus increase the stiffness. Development of an Indentation Model for Fluid Lipid Bilayers. With clean data in place and knowing the potential role of pressure, we set out to generate a quantitative model. Prior nanoindentation experiments of vesicles have been interpreted using the thin elastic shell model.18,19 Elastic shell theory, however, does not account for membrane fluidity, as it assumes a finite in-plane shear modulus. Therefore, we introduce a model based on the Canham−Helfrich theory for fluid bilayer membranes.27,31,32 This theory has been widely used for description and characterization of membranes in a variety of experimental studies, mostly at the micrometer scale.16,17 In our model, we use symmetric bilayers with a bending modulus κ. We model a nanoindentation experiment as compression between two tips, which we do for two reasons. On the one hand, one may expect that deformation occurs mostly near the tip, in which case the deformation of one hemisphere in the symmetric case can be used to approximate the deformation of a hemispherical adherent vesicle. On the other hand, any attempt to model the adhesion more directly, would require knowledge of the adhesion strength, which we lack. Following Seifert et al.,32 we characterize the (assumed axisymmetric) vesicle by a coordinate S, where 0 ≤ S ≤ S1, and angle ψ (S), as well as Cartesian coordinates x(S) =

∫0

Figure 3. Theoretical force indentation response based on Canham−Helfrich theory. (a) Parametrization of the model. An undeformed (solid black sphere) and deformed shape (dashed line) are shown. Z is the axis of symmetry. S is the length of the arc, which is zero at the “South Pole” and maximum at S1. The angle ψ(S) is the angle between the contour and the x-axis at point S. (b) Theoretical indentation curve for reduced pressure (ΔΠRc3κ−1) 1800, for a parabolic tip with Rt = 0.1 Rc (solid line). In regime I (blue background), the apex of the vesicle flattens and the force response curve is slightly superlinear. In regime II (green) the response softens and a tether is formed. In regime III the response stiffens due to increased contact area between vesicle and tip. Dashed and dotted line show indentation curves with Rt = 0.25 Rc respectively Rt = 0.5 Rc. At the top shapes belonging to the 3 different regimes (indentations 0.2, 0.55, and 0.87 Rc from left to right) are visualized (arrows indicate axes in x,y, and z-direction). Lower right inset shows same curves on logarithmic scale (units same as main panel). (c) Inflection point determination. Upper panel shows a typical force distance curve illustrating the experimental determination of the inflection point for a 200 nm vesicle. FDC with ∼1000 points (in gray); smoothed FDC (in black); numerical derivative of FDC (blue line). Peak of derivative corresponds to the inflection point. Lower panel shows histogram with the localization of inflection point for 200 nm vesicles. Twenty-six out of 34 FDCs (∼76%) that do not show discontinuities before 0.3 Rc were used. Red arrow indicates predicted theoretical value.

S

cos ψ (S′)dS′

(1)

and a similar expression for z (S) with cos ψ replaced by sin ψ. The origin is chosen to be the “South Pole” (Figure 3a). We impose the following conditions for a closed membrane: ψ (0) = 0, ψ (S1) = π, and x(0) = x(S1) = 0. In these terms, the free energy associated with bending is F = 2πκ

∫0

S1 ⎛ x ⎡

⎜ ⎢ψ ̇ + ⎝2⎣

⎤2 ⎞ sinψ − c0 ⎥ ⎟dS ⎦⎠ x

(2)

where c0 is the spontaneous curvature. We use zero spontaneous curvature and note that our results are insensitive to a spontaneous curvature on the order of the vesicle radius (Figure S8). Since the applied force is perpendicular to the bilayer plane, the contribution of stretching is expected to be negligible, and we assume the membrane to be laterally incompressible. We impose this constraint by the condition of constant area: 4πR c2

= 2π

∫0

S1

Since this constraint reduces to a choice of S1 for a given geometric shape defined by ψ, we choose to simply define ψ to be a function of σ = S/S1 ∈ [0,1] (Supporting Information). Using this approach, e.g., for symmetric vesicle shapes, we define ψ (σ) as a sum over various shape modes: nmax

xdS

ψ (σ ) = πσ + (3)

∑ ansin(nπσ ) n=1

2631

(4) DOI: 10.1021/acsnano.6b07302 ACS Nano 2017, 11, 2628−2636

Article

ACS Nano We choose to use only the first six shape modes (n = 6) (Supporting Information, Figure S9). To model an applied indentation force acting at the “North Pole”, we add an additional term to the energy F of the form fz(S1). This approach corresponds to symmetric, point-like tips indenting the vesicles from both poles if only even shape modes an are allowed to be nonzero. We implemented symmetric parabolic tips of curvature Rt by the addition of a potential U0

∫ dA max(0, −R tx 2 − z)

Moreover, this is in agreement with recent MD-simulations showing flattening of the FDC at similar indentations.9 (III) Finally, the finite size of the AFM tip prevents tether extension and leads to a tip dominated stiffening (α ≈ 2) (Figure 3b). Corresponding shapes to the three regimes are shown as insets in Figure 3b. A larger tip results in an earlier onset of the stiffening (Figure 3b) and an extended deformation zone of the vesicle (Figure S10). However, at low pressures no tether forms and, instead, deformation occurs on longer length scales, which results in the tip size dependence becoming apparent only at deeper indentations (Figure S8). Hence, the experimental observation of tip dependence for small indentations (Figure 2d) suggests that the vesicles are strongly pressurized. Furthermore, softening of experimental FDCs occurs at similar indentation as in the model at 0.31 ± 0.03 Rc (s.e.m., N = 26) (Figure 3c). Together, this shows that the model accurately describes the experimental results and that vesicles in our experiments are likely strongly pressurized. Bending Modulus and Pressure Estimation. Finally, to understand the mechanical response of our vesicles, we need to take pressurization into account. Osmotic pressurization occurs when a vesicle is deformed on the surface in our experiments. However, it is probably a biologically relevant effect, since other interactions, such as adherence of vesicles to a cell surface, likely result in similar pressurization. Experimentally, we estimate the pressure from outward membrane tethers formed during retraction of the AFM tip (Figure 4a, Figure S11). It is

(5)

to the energy, again, provided that only even modes an are allowed. There, the strength U0 of the potential is simply chosen to be large enough to enforce that z > − Rtx2/2, which can only affect the lower hemisphere. However, due to the use of only even modes an, this condition is also imposed on the upper hemisphere. Finally, a pressure difference is included. It is necessary to account for two distinct contributions, the luminal osmotic pressure Πint and the external osmotic pressures Πext, where the former increases with decreasing volume

V=π

∫0

S1

x 2 sin ψdS

(6)

during indentation, while the latter is constant. Given a net pressure difference ΔΠ = Πint − Πext, the change in free energy is given by dF = −ΔΠdV. We assume a dilute solution (ideal gas) form for the internal pressure Π int =

(0) Π(0) int V V

(7)

where (0) refers to prior to indentation. To solve for the vesicle shape, we minimize the full energy, including bending, pressure, and tip shape, for a given force f, subject to the various constraints, including the area constraint. This yields the various shape amplitudes an, as well as the length S1. From these, we obtain the height z(S1) and indentation, as functions of the applied force f. Solving the shape for various forces then allowed construction of theoretical FDCs (Figure 3b). By working in reduced coordinates x̂ and ẑ, it becomes natural to express energies in units of 2πκ, lengths in units of πRc, forces in units of 2κR−1 c , −3 stiffness in units of 2κR−2 c , and pressure in units of πκRc . In this model of a symmetric vesicle, the mechanical response depends only on a single unknown, the bending modulus, along with ΔΠ and the AFM tip radius, which can both be determined separately. Experimental Observations Agree Well with the Model for Fluid Lipid Bilayers. The indentation response (Figure 3b) based on our model exhibits three regimes: (I) an approximately linear (exponent α ≈ 1.05) increase of force with indentation that corresponds to the flattening of the apex of the vesicle. The stiffness K for small indentations (