er, Production of Antisera for Radioim munoassays, in "Principles of Competi tive Protein-Binding Assays," W. D. Odell and W. H. Daughaday, Eds., pp 57-88, Lippincott, Philadelphia, Pa. 1971. 25. J. Vaitukaitis, J. B. Robbins, E. Nieschlag, and G. T. Ross, A Method for Producing Specific Antisera with Small Doses of Immunogen, J. Clin. Endocrinol. Metab., 33, 988 (1971). 26. G. E. Abraham and P. K. Grover, Covalent Linkage of Hormonal Haptens to Protein Carriers for Use in Radioimmu noassay, in "Principles of Competitive Protein-Binding Assays," W. D. Odell and W. H. Daughaday, Eds., pp 134-57, Lippincott. Philadelphia, Pa., 1971.
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Radiolabeled Antigen 27. R. S. Yalow and S. A. Berson, Label ing of Proteins—Problems and Prac tices, Trans. Ν. Υ. Acad. Sci., 28, 1033 (1966). 28. A. E. Freedlender, Practical and The oretical Advantages for the Use of I 125 in Radioimmunoassay, in "Protein and Polypeptide Hormones," M. Margoulies, Ed., pp 351-3, Excerpta Med. Int. Congr. Series No. 161, Amsterdam, the Netherlands, 1968. 29. G. C. Oliver, Jr., B. M. Parker, D. L. Brasfield, and C. W. Parker, The Mea surement of Digitoxin in Human Serum by Radioimmunoassay. J. Clin. Invest., 47,1035(1968). 30. A. L. Steiner, D. M. Kipnis, R. Utiger, and C. Parker, Radioimmunoassay for the Measurement of Adenosine-3'5'Cyclic Phosphate, Proc. Nat. Acad. Sci., U.S., 64,367(1969). 31. W. T. Newton, J. E. McGuigan, and Β. Μ. Jaffe, Radioimmunoassay of Pep tides Lacking Tyrosine, J. Lab. Clin. Med., 75,886(1970). 32. W. M. Hunter, The Preparation and Assessment of lodinated Antigens, in "Radioimmunoassay Methods," Κ. Ε. Kirkham and W. M. Hunter, Eds., pp 3-23, Churchill Livingstone, Edinburgh, Scotland, 1971. Incubation, Separation of Bound and Free-Labeled Antigen 33. C. N. Hales and P. J. Randle, Immu noassay of Insulin with Insulin Antibody Precipitate, Biochem. J., 88, 137 (1963)" 34. V. Herbert, K. S. Lau, C. W. Gottlieb, and S. J. Bleicher, Coated Charcoal Im munoassay of Insulin, J. Clin. Endocri nol. Metab., 25, 1375 (1965). 35. A. M. Eisentraut, N. Whissen, and R. H. linger, Incubation Damage in the Radioimmunoassay for Human Plasma Glucagon and Its Prevention with Trasylol, Amer. J. Med. Sci., 255, 137 (1968). 36. K. J. Catt and G. W. Tregear, Solid Phase Radioimmunoassay, in "Protein and Polypeptide Hormones," M. Mar goulies, Ed., pp 45 8, Excerpta Med. Int. Congr. Series No. 161, Amsterdam, the Netherlands, 1969. 37. D. Rodbard, H. J. Ruder, .]. Vaitukai tis, and H. S. Jacobs, Mathematical Analysis of Kinetics of Radioligand As says: Improved Sensitivity Obtained by Delayed Addition of Labeled Ligand, J. Clin. Endocrinol. Metab., 33, 343 (1971). 38. W. H. Daughaday and L. S. Jacobs, Methods of Separating Antibody-Bound from Free Antigen, in "Principles of Competitive Protein-Binding Assays," W. D. Odell and W. H. Daughaday, Eds., pp 303-24, Lippincott, Philadel phia, Pa., 1971. Methods Related to Radioimmunoassay 39. B. P. Murphy and C. J. Pattee, Deter
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mination of Thyroxine Utilizing the Propertv of Protein-Binding, J. Clin. Endocrinol. Metab., 24, 187 (1964). 40. Β. Ε. P. Murphy, Some Studies of the Protein-Binding of Steroids and Their Application to the Routine Micro and Ultramicro Measurement of Various Steroids in Body Fluids by Competitive Protein-Binding Radioassay.e/. Clin. Endocrinol. Metab., 27, 973 (1967). 41. S. P. Rothenburg, A Radioenzymatic Assay for Folic Acid, Nature, 206, 1154 (1965). 42. R. J. Lefkowitz, J. Roth, and I. Pastan, Radioreceptor Assay of Adrenocort icotropic Hormone: New Approach to Assay of Polypeptide Hormones in Plas ma, Science, 170,633(1970). 43. L. E. M. Miles and C. N. Hales, The Use of Labeled Antibodies in the Assay of Polypeptide Hormones, J. Nucl. Biol. Med., 13, 10(1969).
C h a r l e s D . H a w k e r is chief clinical chemist and director of research a n d development for Laboratory Proce dures®, a division of T h e Upjohn Co., K a l a m a z o o , Mich. Laboratory Proce dures is a nationwide clinical labora tory network. Dr. Hawker earned a BA degree in chemistry from Illinois Wesleyan University (Bloomington) in 1962, an M S degree in biochemis try from t h e University of Wisconsin (Madison) in 1965, a n d a P h D in bio chemistry from t h e University of Pennsylvania (Philadelphia) in 1967. H e was the recipient of an N I H post doctoral fellowship from 1967-69, and from 1967-71 engaged in research on the r a d i o i m m u n o a s s a y of p a r a t h y r o i d hormone at the University of P e n n sylvania. He has been with L a b o r a t o ry Procedures since 1971 and is di recting the d e v e l o p m e n t of radioim munoassays for m a n y c o m p o u n d s in cluding protein hormones, steroid hormones, a n d drugs. Dr. Hawker is a m e m b e r of P h i L a m b d a Upsilon, the American Chemical Society, the American Association for t h e Ad v a n c e m e n t of Science, the American Federation for Clinical Research, a n d the Endocrine Society.
