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Corn Silk Extract and Its Bioactive Peptide Ameliorated LipopolysaccharideInduced Inflammation in Mice via Nuclear Factor-#B Signaling Pathway Tin-Yun Ho, Chia-Cheng Li, Hsin-Yi Lo, Feng-Yuan Chen, and Chien-Yun Hsiang J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b03327 • Publication Date (Web): 08 Jan 2017 Downloaded from http://pubs.acs.org on January 11, 2017
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Corn
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Lipopolysaccharide-Induced Inflammation in Mice via Nuclear Factor-κB
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Signaling Pathway
Silk
Extract
and
Its
Bioactive
Peptide
Ameliorated
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Tin-Yun Ho,†,‡,a, Chia-Cheng Li,†,a, Hsin-Yi Lo,†, Feng-Yuan Chen,† and Chien-Yun
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Hsiang*,§
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†
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Taiwan ‡
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Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402,
Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan
§
Department of Microbiology, China Medical University, Taichung, Taiwan
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*
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Hsueh-Shih Road, Taichung 40402, Taiwan. Tel.: +886 4 22053366 x 2163. Fax:
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+886 4 22053764. E-mail address:
[email protected] Corresponding author. Department of Microbiology, China Medical University, 91
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a
These authors contributed equally to this work.
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Short title: Anti-inflammatory effects of corn silk and its bioactive peptide 1
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Bioactive peptides derived from foods have shown beneficial anti-inflammatory
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potential. Inhibitory κB kinase-β (IKKβ) plays a crucial role on the activation of
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nuclear factor-κB (NF-κB), a transcription factor involved in inflammation. Here we
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applied proteomic and bioinformatics approaches to identify anti-inflammatory
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peptides that target IKKβ from corn silk. Corn silk extract significantly suppressed
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lipopolysaccharide (LPS)-induced NF-κB activities (1.7±0.2 fold vs. 3.0±0.6 fold,
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p
