Correction to “Comparison of Different Sample Preparation Protocols

Addition/Correction pubs.acs.org/jpr

Correction to “Comparison of Different Sample Preparation Protocols Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria and Human Cells” Timo Glatter,* Erik Ahrné, and Alexander Schmidt* J. Proteome Res. 2015, 14, pp 4472−4485. DOI: 10.1021/acs.jproteome.5b00654

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n Figure 1 the chaotropic salt concentration for the trypsin digestion step was mislabeled. The correct salt concentrations (1.6 M urea, 1.6 M Gua, and 1.4 M urea/0.4 M Gua) are given in the main text throughout the manuscript.

Figure 1. Quantitative comparison of solubilization and digestion efficiencies using different sample preparation strategies. Pseudomonas aeruginosa and human embryonic kidney 293 (HEK) cells were lysed using the indicated buffer conditions. After measuring the extracted total protein amount by BCA, 50 μg of protein was used for protein digestion. Upon tandem LysC/trypsin digestion LC−MS analysis, label-free quantification and statistical evaluation of quantification results by SafeQuant were performed. All significantly regulated proteins were then subjected to analysis by the functional annotation tool DAVID. ISD, in-solution digestion.

© 2015 American Chemical Society

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DOI: 10.1021/acs.jproteome.6b00012 J. Proteome Res. XXXX, XXX, XXX−XXX