Correction to Stable Sulfur and Oxygen Isotope Fractionation of Anoxic

Addition/Correction pubs.acs.org/est

Correction to Stable Sulfur and Oxygen Isotope Fractionation of Anoxic Sulfide Oxidation by Two Different Enzymatic Pathways Carsten Vogt,* Alexander Poser, and Hans-Hermann Richnow Environ. Sci. Technol. 2014, 48 (16), 9094−9102; DOI: 10.1021/es404808r

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n our recent article,1 the study of Beller and colleagues2 is used as a reference to state that Thiobacillus denitrificans preferentially employs the sulfide: quinone oxidoreductase (SQR) pathway over the Sox pathway under denitrifying conditions. However, the data of Beller and colleagues2 do not support this statement, as they show that genes of both pathways (SQR and Sox) are highly expressed under denitrifying conditions. Our general conclusion that T. denitrificans predominantly uses the SQR pathway for oxidizing sulfide to sulfate under denitrifying conditions is still valid, as Beller and colleagues showed in a separate study3 that T. denitrificans contains all genes of the SQR pathway, but does not contain soxCD of the Sox pathway, indicating that T. denitrificans cannot oxidize zerovalent sulfur to sulfate by the Sox pathway. We thank Harry R. Beller for helping us in correcting our discussion.

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REFERENCES

(1) Poser, A.; Vogt, C.; Knöller, K.; Ahlheim, J.; Weiss, H.; Kleinsteuber, S.; Richnow, H. H. Stable sulfur and oxygen isotope fractionation of anoxic sulfide oxidation by two different enzymatic pathways. Environ. Sci. Technol. 2014, DOI: 10.1021/es404808r. (2) Beller, H. R.; Letain, T. E.; Chakicherla, A.; Kane, S. R.; Legler, T. C.; Coleman, M. A. Whole-genome transcriptional analysis of chemolithoautotrophic thiosulfate oxidation by Thiobacillus denitrif icans under aerobic versus denitrifying conditions. J. Bacteriol. 2006, 188 (19), 7005−7015. (3) Beller, H. R.; Chain, P. S. G.; Letain, T. E.; Chakicherla, A.; Larimer, F. W.; Richardson, P. M.; Coleman, M. A.; Wood, A. P.; Kelly, D. P. The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitrificans. J. Bacteriol. 2006, 188 (4), 1473−1488.

Published: August 8, 2014 © 2014 American Chemical Society

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dx.doi.org/10.1021/es503808k | Environ. Sci. Technol. 2014, 48, 9958−9958