7966
Biochemistry 1995,34, 7966
Corrections Calorimetric Analysis of the Binding of Lectins with Overlapping Carbohydrate-Binding Ligand Specificities, by Mary C . Chervenak and Eric J. Toone*, Volume 34, Number 16, April 25, 1995, pages 5685-5695. Due to a publishing error, Table 5 and revised Figures 2 and 8 did not appear in the printed edition of the Journal. In addition, the last sentence of the caption to Figure 8 should read as shown below. Also, the following should be included under References: Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946. The table and revised figures should appear as follows :
Table 5: Enthalpies of Binding Attributable to Changes in Solvation AHsolvh
fl
carbohydrate"
con A
Dioclea
aMeMan 1-6 diman 1-3 diman triman
-2.5 -5.5 -2.2 -4.7
-2.8 -2.0 -1.1
-4.8
Abbreviations same as Table 3. kcal mol-'.
Time (min) 0.00
66.67
33.33
100.00
133.33
0.00 -
+
c
a 5
-1.14-
.-0
c
0 0
-
-2.27-3.41 -
:
a
0 Y
-4.55-
FIGURE 8: Two views of concanavalin A showing the similarities between concanavalin A and the lectin from Dioclea. The C a backbone from the Protein Data Bank file 2CTV is shown in dark Injection Number gray at areas of difference (Hardman & Ainsworth, 1972; Ainouz et al., 1987). The lighter sphere is Mn2+, and the darker sphere is FIGURE 2: Calorimetric titration of native Dioclea grandiflora Ca2+. Protein diagrams rendered by MolScript (Kraulis, 1991). (0.3107 mM) with methyl 6-0-(a-~-mannopyranosyl)-a-D-mannopyranoside 14 (14.6 mM) at 15.0 "C: (A) raw data for 40 2.2pL injections of 14, and (B) the integrated curve showing experimental points (.) and the least squares fit (-) to the integrated data. The buffer was 50 mM Na2HP04containing 250 mM NaCl, B19534230 1 mM MnC12, and 1 mM CaC12 at pH 7.15. I
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0006-2960/95/0434-7966$09.00/0 0 1995 American Chemical Society
