CYANOGESIC PL,WTS FROM ARGENTINA DAT-ID P. SEIGLER' Departvietit of Bofaii.,
The L-iiiL'ersi!?
os Z!litiois,
L-rbnna, I!'?iTiois
JORGED. C o r s s ~ oand R C B ~ ST-. D. ROSDIS.~ Cbfedrtr de F a r ~ n a c o g n o s i a Fflcitl!ad , de Farinncia Bioqiiiniica, 1ki;ersidad d e Bitetios .&res, Jiiniti 956, Biietios A i r e s , .Irgeiiiina
.hsrR.wr.-Forty-six samples (thirty-nine species) of primarily Argentine plants produccd a positive test for hydrogen c p n i d e (Guignard). Of these, approximately 25 have not previously been reported as cyanogenic.
Because of their toxicity, economic importance. and xi-ideqpread occurrence (1-S), n-e undertook the present ztudy of cyanogenic plants of the Argentine flora. These initial results are based on the Guignard test. K e are currently attempting to isolate and characterize several of the compounds responsible for cyanogenesiq. \Ye tested a number of plant that nere available to us from materials in the CBtedra de Farmacognoqia, the Jardin Bot Anico "Carlos Thays", the Instituto lliguel Lillo, and from field trips to Tucumfin and Corrientes. T'oucher specimens of these are deposited in the University of Illinois Herbarium. The results of these te-ts are given in table 1. Of approximately 300 .ample. tested, 46 repre.enting 39 +pecies nere positive. We have not included data for negative test. as positive ones are more indicative of the plants genetic potential, i.e.. TI hat biosynthetic p a t h a) exiqt and can be operative. I n .Argentina. the major familie. n hich contain cyanogenic plants ieem t o be the Granlineae. Euphorbiaceae, Leguniiiiosae and Sapindaceae.
.
A\CKSOilL E D G l I E S T h T h e senior author x-ishes to thank the International Commission for the Exchange of scholars lor a Fulbright Ha) s Lecture Research grant. Field trips in the provinces ot Tucnnian and Corrientes x e r e made possihle b) Dr F 1-ervoorst, Dr P. beeligmann, D r . -1. Krapovickas, C. Krapovickas and D r Italo Ricciardi The director and personnel of the Jardin Botanico 'Carlos T h q s and of the botanical gardens of ISTa\ in Castelar, Pcia. Buenos Aires, graciously made their facilities available R e also xis11 t o thanh Dr A4rturoRagunese for his cornnientq and suggestions concerning poisonous plant- of hrgentinn. 'Fulbright Ha?-s Lecturer Researcher in the Catedra de Farmacognosia, July 1976December 1976. 179
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LITERATURE CITED
E. E. COPIN,Biociieni. Synrp., 38, 277 (1973). D. S. SEIGLER,Phytochemistry, 14, 9 (1975). E. E . C o n and G. W. BUTLER, I n ,J. B. HARBORPIE and T . SWAIS (eds.)? “Perspectives in
Phytochemistry,“ Academic Press, London, 1969.
4. R . EYJ~LFSSOX, Fort. Cheni. O r g . Xa/urst., 27, 74 (19il). 5 . E. E. COSN, In “Toxicants Occurring Naturally in Foods,” 2nd ed., Committee on Food
Protection, National Research Council, Washington, D.C., 19T4. 6. K. L. MIKOL.~JCZAK, Prog. C h e w . Fats other Lipids, 15, 97 (1977). 7. D. S. SEIGLER, In L. REINHOLD, J. B . HIRBORSEand T. SWAIN (eds.), “Progress in PhytoPhytochemistrJ-,’ Yol. 4, Pergamon Press, Oxford, 197T. 8. J. 11.KISGSBURP,“Poisonous Plants of the U.S. and Canada,” Prent,ice Hal!, Englewood Cliffs, Kew Jersey, 1964. 9. N . G . Asrusso, Rea. Insest. Agropec. I S T A , Serie2, Biol. Prod. Veg., 3, S o . 22, 347 (1966). 10. L . R . PIRODI,Rev. Argentina Agron., 17 (3) 163 (1950). 11. E. L. R.ITER.I, Cienc. e Inzest. 1 (9) 434 (1945). 12. -4.E. RAGOSESE, Reo. Fac. Agron., La Pluitta, 38 Epoca, 31, 1 (1971). 13. E. L. R.\TER.I, Ing. Agron., Ju1.-Ago., No. 4, 1 (1954). 14. A. C. LBEMAS,Onderstepoorf. J . Vet. Sci. &? .4nini. I d . , 5 , 97 (1935). 15. G. COT-AS and C. D. ITRIA, Dir. Gen. de Invest. Agric. Comm. I-.Anivers., pgg. 21, Castelar, Pcia. Buenos Aires, Argentina (1949). Bol. Dir. d e M e d i c . V e ! e r . Pro!,. d e Bzrenos A i r e s , La Plata, 5 (23 and 16. DIEGODE ALBERTO, 26) 46 11948). 17. A.’GcRNI. &a. F U C .Agron., 50, 1, 73 (1974). 18. E. A. LERA,GQC.V e ! . , Bztenos Aires, 7 (34) 66 (1945). 19. P. Y. SCHASG and 11.C. AR.AMEKDI, GQC.Vet., Buenos Aires, 6 (31) 264 (1944). 20. N . G. ABICSSO,I D I A , K O . 193, Buenos Aires, 14 (1964). 21. E. -4.H.ICSX-IRTH, Re;,. A g V o r i . A-orocst. Arg., 2, 5 (1956). 22. M. E. BOMAN, Bull. d i i Jluskzrt77 d’Hist. A7atztr. Annee‘, KO.5 . 337 (1905). 23. J. PELISCH, T e s i s Ddo. Quint. Fuc. Cienc. Exact. Fis. S a t . Bzrenos AireT, Cfr. Trab. Inst. Bot. Fern?.41, 1 (1920). 24. R . GBIELIN, 11.S C H ~ L Eand R E. BORDAS, Piiytochemistry, 12, 457 (1973). 25. H . FISSEIIORE, 8 . K. REICHARD and D. K. L-ARGE, J . Proc. Roy. SOC. N S W , 70, 209 (1938). 26. L. T. S.FAT,Konikl. -V:ederlaxdseAkad. TT’etensch., 80 C , 227 (1977). 27. A. J. BANDOXI, Arg. Rez. Farni., 78, 171 (1936). 28. R . TT. D. RONDISA,hI. E. hfENDIOND0, and J. D. Cocssro, Rte. Invesf. Agropec., ILYTA, Buenos Aires, 7 , 271 (1970). 29. G . TAPITISEWIE, H. R.L. RUIJGROK and R. HEGSACER, Pharm. Weekbl., 104, 1341 (1969). Piiarni. Tl‘eekbl., 93, 801 (1958). 30. R . HEGPIAUER, EMORE, J. If. COOPERand L. J. HARRIS,J . SOC.Chem. Ind. 57, 162 (1938). 31. EMORE and J . H. COB-CRAFT. J . Soc. C h e m . Ind.. Trans.. 57. 166 (1938). 32. Tesis , Fuc. d e Cien. Ex&. Fis.X a t . Vol. 1,‘BuenosAiies (1901)l’ 33. H. D U C L A U X C. R. Acad. Sci. Paris, 154, 1249 (1912). 34. H. HBRTSSEY, 35. D. S. SEIGLER,Biocheni. Syst. & Ecol., 4, 235 (1976) Biochem. Syst. b Ecol., 4, 263 (1976). 36. D. S. SEIGLERand ITr. K.AKAHAR.A, 37. D. S. SEIGLER, C. EGGERDISG and C. RCTTERFIELD, Phytochemistry, 13, 2330 (1974). 38. K . L. ~ ~ I K O L I J C Z A Kc, . R . SMITH,J R . and L. R.T J A R K S , Lipids, 5 , 812 (1970). 39. A. E . RAGOKESE, Publicacion TBcnica KO.82. (Yueva Serie) Direccion de Investigaciones .4gricolas, Instituto de BotAnics Agrico!a, Buenos Aires (1956).
