Article pubs.acs.org/ac
Design of Peptide-Based Probes for the Microscale Detection of Reactive Oxygen Species Chun-Lan Keng,† Ying-Chi Lin,‡ Wei-Lung Tseng,§ and Chi-Yu Lu*,†,∥,⊥,# †
Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan § Department of Chemistry, College of Science, National Sun Yat-sen University, Kaohsiung 80424, Taiwan ∥ Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ⊥ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan # Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan ‡
ABSTRACT: Reactive oxygen species (ROS) can induce oxidative stress and are associated with cell death and chronic diseases in organisms. In the treatment of disease, drugs that induce ROS are associated with many side effects and unpleasant symptoms. Therefore, during the assessment of new drugs and candidate compounds, ROS generation is an issue of concern, because ROS can modify proteins, lipids, and nucleic acids within organisms and alter their biological functions. In this work, we designed a peptide-based probe for the rapid (
