J. Agric. Food Chem. 1988,36,863-870
863
Development of an Enzyme-Linked Immunosorbent Assay for the Analysis of the Thiocarbamate Herbicide Molinate Shirley J. Gee, Toru Miyamoto,' Marvin H. Goodrow, Dan Buster, and Bruce D. Hammock* The development of an enzyme-linked immunosorbent assay (ELISA) for the herbicide molinate (Ordram, S-ethyl hexahydroazepine-l-carbothioate)is described. By a thiol replacement reaction of thiocarbamate sulfones, several haptens were synthesized. These haptens were coupled to carrier proteins via mixed-anhydride and diazotization procedures. Antibodies raised against these antigens were screened for sensitivity and specificity for molinate. An assay is described with a limit of detectability of 3 ng/mL that is specific for molinate with some cross-reactivity (15%) to molinate sulfone. Other thiocarbamate pesticides had cross-reactivities of
