Development of an Immunoassay (ELISA) for the Quantification of

J. Agric. Food Chem. 2001, 49, 1675−1680

1675

Development of an Immunoassay (ELISA) for the Quantification of Thiram in Lettuce Anne-Laurence Queffelec,† Frederic Boisde´,‡ Jean-Pierre Larue,† Jean-Pierre Haelters,‡ Bernard Corbel,‡ Daniel Thouvenot,† and Patrice Nodet*,† Laboratoire de Microbiologie et Se´curite´ Alimentaire, ESMISAB, Technopoˆle Brest-Iroise, 29280 Plouzane´, France, and Laboratoire de Chimie He´te´ro-Organique, FRE 2125 CNRS-UBO-MNHM, UFR Sciences et Techniques de Brest, B.P. 809, 29285 Brest Cedex, France

Two competitive immunoassays, a laboratory assay based on microwell plates and a field test based on the use of polystyrene tubes, have been developed for the quantification of thiram in lettuces. Concerning the laboratory assay, the calibration curve for thiram had a linear range of 11 to 90 ng/mL and a detection limit of 5 ng/mL. Precision of the assay presented coefficient of variation values