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Comparative analysis of EPA/DHA-PL forage and liposomes in orotic acid-induced non-alcoholic fatty liver rats and their related mechanisms Mengru Chang, Tiantian Zhang, Xiuqing Han, Qingjuan Tang, Teruyoshi Yanagita, Jie Xu, Changhu Xue, and YuMing Wang J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b05173 • Publication Date (Web): 18 Jan 2018 Downloaded from http://pubs.acs.org on January 19, 2018
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Comparative analysis of EPA/DHA-PL forage and liposomes in
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orotic acid-induced non-alcoholic fatty liver rats and their
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related mechanisms
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Mengru Changa, Tiantian Zhanga, Xiuqing Hana, Qingjuan Tanga, Teruyoshi Yanagitac, Jie Xua,
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Changhu Xueab, Yuming Wangab,*
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a
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Qingdao, Shandong Province 266003, PR China
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b
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Science and Technology, Qingdao, Shandong Province, PR China
College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road,
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine
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c
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*Author (Yuming Wang) for correspondence (E-mail:
[email protected]; Phone:
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0532-82032597; Fax: 0532-82032468)
Department of Health and Nutrition Sciences, Nishikyushu University, Kanzaki 842-8585, Japan
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Non-alcoholic fatty liver disease (NAFLD) has become one predictive factor of death from
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various illnesses. The present study was to comparatively investigate the effects of
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eicosapentaenoic acid-enriched and docosahexaenoic acid-enriched phospholipids forage (EPA-PL
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and DHA-PL) and liposomes (lipo-EPA and lipo-DHA) on NAFLD and demonstrate possible
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protective mechanisms involved. The additive doses of EPA-PL and DHA-PL in all treatment
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groups were 1 % of total diets, respectively. Results showed that Lipo-EPA could significantly
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improve
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aminotransferase (AST) and alanine aminotransferase (ALT) levels by 55.6 % and 34.2 %,
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respectively (p < 0.01). Moreover, lipo-EPA exhibited excellent inhibition on the mRNA
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expression of SREBP-1c and FAS at the values of 0.454 ± 0.09 (p < 0.01) and 0.523 ± 0.08 (p
