Anal. Chem. 2008, 80, 3762–3768
Drug Assessment Based on Detection of L-Glutamate Released from C6 Glioma Cells Using an Enzyme-Luminescence Method S. M. Zakir Hossain,*,† Hiroaki Shinohara,*,‡,§ and Hiromi Kitano†,| Course of Advanced Nano and Biosciences and Course of Biological Information Systems, Graduate School of Innovative Life Science for Education, and Field of Life, Information and System Sciences and Field of Nano and Functional Material Sciences, Graduate School of Science and Engineering for Research, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan Monitoring of excitation activity of nerve cells is very useful for not only brain research but also assessment of the effects of various chemicals, including drugs and toxins.Wepreviouslyreportedanovelenzyme-luminescence method for real-time monitoring of L-glutamate release from C6 glioma cells with high levels of sensitivity (≈10 nM) and temporal resolution (
