J. Agric. Food Chem. 2008, 56, 6609–6615
6609
ELISA for Sulfonamides and Its Application for Screening in Water Contamination WEILIN L. SHELVER,*,† NANCY W. SHAPPELL,† MILAN FRANEK,§ FERNANDO R. RUBIO#
AND
Biosciences Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 1605 Albrecht Boulevard, Fargo, North Dakota 58105; Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic; and Abraxis LLC, 54 Steamwhistle Drive, Warminster, Pennsylvania 18974
Two enzyme-linked immunosorbent assays (ELISAs) were tested for their suitability for detecting sulfonamides in wastewater from various stages in wastewater treatment plants (WWTPs), the river into which the wastewater is discharged, and two swine-rearing facilities. The sulfamethoxazole ELISA cross-reacts with several compounds, achieving detection limits of 1000 0.88
% cross-reactivity
