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Enhanced Selectivity by Passivation: Molecular Imprints for Viruses with Exceptional Binding Properties Manuela Gast, Stefanie Kuehner, Harald Sobek, Paul Walther, and Boris Mizaikoff Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.7b05148 • Publication Date (Web): 05 Mar 2018 Downloaded from http://pubs.acs.org on March 10, 2018

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Analytical Chemistry

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

ASSOCIATED CONTENT Supporting Information ^2&!-%>>73*/$A!=$.73B#*/7$!/)!#E#/'#8'&!.3&&!7.!@2#3A&!7$!*2&! MP-!K%8'/@#*/7$)!D&8)/*&!#$6!/$@'%6&)c! ~C>7*&$*/#'! B&#)%3&B&$*),! E/3%)! 8/$6/$A! )*%6?! E/#! O%#$*/*#*/E&! KPQ,! #$6! 6&*&3B/$#*/7$! #$6! @7$*37''/$A! 7.! *2&! >7'?B&3!'#?&3!*2/@9$&))!HKjNI!

AUTHOR INFORMATION Corresponding Author
Author Contributions ^2&!B#$%)@3/>*!D#)!D3/**&$!*237%A2!@7$*3/8%*/7$)!7.!#''! #%*273)F!

Additional Information M6&$7E/3%)!/)!#!>#*27A&$!*2#*!/)!@#>#8'&!7.!A&$&3#*/$A! /$.'#BB#*73?!/BB%$&!3&)>7$)&)!/$!2%B#$F!"/$%*&!E/3%)!7.! B/@&!/)!7$&!7.!*2&!B7)*!.3&O%&$*!E/3#'!>#*27A&$)!/$!'#873#*73?! B/@&F!M''!D739!D/*2!#6&$7E/3%)!#*!B/$/B%B!3&O%/3&)!:-JC4! @7$*#/$B&$*F!5&$@&,!#''!&R>&3/B&$*)!/$E7'E/$A!#@*/E&! #6&$7E/3%)!#$6!"a"!D&3&!&R@'%)/E&'?!&R&@%*&6!#*!J#873!j3F! "&39!n!07''&A&$!(B85!&O%/>>&6!D/*2!*2&)&!.#@/'/*/&)F!!!

ACKNOWLEDGMENT This work has been supported by the Federal Ministry of Education and Research (BMBF) within the projects PROTSCAV I and II. The authors thank the Electron Microscopy facility and the Focused Ion Beam Center UULM at Ulm University for assistance during these studies.

ABBREVIATIONS KL(,! PP! >7'?&*2?'&$&! A'?@7'v! :-M,! PP! 87E/$&! )&3%B! #'8%B/$v! -M",! PP! )&'.C#))&B8'&6! B7$7'#?&3v! LJ=-M,! PP! &$;?B&C'/$9&6! /BB%$7)738&$*! #))#?v! OKPQ,! PP! O%#$*/*#*/E&! >7'?B&3#)&! @2#/$! 3&#@*/7$v! "=K,! B7'&@%'#3'?! /B>3/$*&6! >7'?B&3v! $7$C/B>3/$*&6! >#3*/@'&,! PP! T=Kv! ^L",! PP! *3#$)B/))/7$! &'&@*37$! B/@37)@7>?v! ^QK-,! PP! *%$#8'&! 3&)/)*/E&! >%')&! )&$)/$Av! a=K,! PP! E/3%)! /B>3/$*&6! >#3*/@'&v! -L",!PP!)@#$$/$A!&'&@*37$!B/@37)@7>?v!-K,!PP!)/'/@#!>#3*/@'&v! MK^L-,! PP! SC#B/$7>37>?'IC*3/&*27R?)/'#$&v! K:-,! PP! >27)>2#*&C8%..&3&6! )#'/$&v! ^L`-,! PP! *&*3#&*2?'73*27)/'/@#*&v! MK^"-,! PP! HSC#B/$7>37>?'IC *3/B&*27R?)/'#$&v! 5P',! PP! 2?637@2'73/@! #@/6v! M6a,! PP! M6&$7E/3%)v! "a",! PP! "/$%*&! a/3%)! 7.! "/@&v! K:-,! PP! K27)>2#*&C:%..&3&6! -#'/$&v! PP-,! PP! @&''! @%'*%3&! )%>&3$#*#$*F!



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REFERENCES H+I!! J&$)&$,!"F!PFv!-@2%'*&,!aF!MFv!j/&;,!"F!=$!2'*5-&)6'-.%";" S4/%'0%"-$1"34)5'%&6/v!=$^&@2c!J7$67$,!4\++v!>>!S[YV U+UF! H4I!! :3%/$)B#,! (F! "Fv! E#$! 6&3! "&/,! 5F! PFv! :%))@2&3,! 5F! tF! :#@*&3/#'!2'*5-&)6'-.%!AKK5,!AA!H4UI,!S4+YVS44UF! HSI!! ^/33&'',!"Fv!07997'/,!LFv!:/&)#')9/,!"F!P(6+D"P0'D!AKKA,!U??,! W+VZSF! HUI!! -2&$,! "Fv! G#A$&3,! "F! -Fv! P#)*$&3,! jF! (Fv! Q#*$&3,! :F! jFv! 5738&**,!^F!MF!B-$I5('6!AKKD,!!>,!+W[4V+W[[F! H]I!! Q7#@2,!KFv!N#33#3,!jFv!K&33?,!PF!PF!VD",5D"34)5D"P*0D!AKK?,! !AW,!Z+WZVZ+YSF! HWI!! P2#>B#$,!QF!(Fv!`)*%$/,!LFv!^#9#?#B#,!-Fv!57'B'/$,!QF!LFv! •#$,!JFv!G2/*&)/6&),!(F!"F!!VD",5D"34)5D"P*0D!AKKK,!!AA,! ZS\SVZS\UF! HYI!! P2&$A#,! (Fv! €2#$A#,! €Fv! P2&$#,! -Fv! :3?&3)8,! t#B&)! jF! 2'*5-&)6'-.%!AKKU,!A=,!U+[4VU+[[F! HZI!! Q7)@2&,! :Fv!J/,!hF!€Fv!5#%&3,! :Fv! -@2B/6,!MFv! :%&2'&3,! 0F! X6)$1%"2'*&)04$*.D!AKKY,!AW,!WSWVWUSF! H[I!! Q#*$&3,! :Fv! 57..B#$,! MFv! -@27&$,! NFv! J&B7$),! tFv! /$! 2'*5-&)6'-.%" P0')$0)Q" ,$" #$&6*1(0&'*$" &*" E-&)6'-.%" '$" E)1'0'$)v!Q#*$&3,!:F!jF,!L6Fv!M@#6&B/@!K3&))c!-#$!j/&A7,! +[[WF! H+\I!! 5'#6?,!aFv!:%/d),!tF!3(66D"LR'$D"2'*&)04$*.D!5YYL,!W,!Y4V YYF! H++I!! "39)/@2,!"F!34)5D"P*0D"O)9D!AKKK,!A>,!4WYV4YSF! H+4I!! `)*%$/,!LFv!P2#>B#$,!QF!(Fv!57'B'/$,!QF!LFv!^#9#?#B#,! -Fv!G2/*&)/6&),!(F!"F!B-$I5('6!AKK5,!!W,!]W\]V]W4\F! H+SI!! P2#$63#67)),!-F!jFv!5##A)B#,!MF!PFv!J&&,!•F!0Fv!5D#$A,! tFC5Fv!T#B,!tFC"Fv!t77,!PF!VD"Y'%D">,! U4ZUVU4Z[F! H4\I!! 5&?&),! PF! jFv! 078/*)9/,! MF! •Fv! MB/3A7%'7E#,! LF! aFv! T/&$2#%),! (F! eF! VD" S4/%D" 34)5D" 2! AKKH,! !?=,! +SSZYV +SS[UF! H4+I!! T/'))7$,!QFv!-doA3&$,!5F!`F!VD"#55($*.D"E)&4*1%!5Y@H,! [[,!+YV4]F! H44I!! j%'8&@@7,!QFv!a7A*,!"F!3*.1"PR6'$I"J-6:D"P/5RD"^(-$&D" 2'*.D!5Y?D,!!=,!4YSV4Y[F! H4SI!! G5`! C! G73'6! 5&#'*2! `3A#$/;#*/7$v! N#@*! )2&&*! TpS+\v!4\+UF! H4UI!! 0%'/@2,! KF! MFv! ^#?'73,! jF! JFv! MB&3/@#$! -7@/&*?! .73! "/@378/7'7A?F!X4)"#$+)0&'*$"S6)9)$&'*$'%&_%"@('1)"&*"&4)" B-:v!4\+4F! H4]I!! -*&>2&$)7$,! tF! QF,! G#3$&),! M! /$" C'-I$*%&'0"Y'6*.*I/" S6*&*0*.%,!4$6!&6Fv!->3/$A&3!>37*7@7')v!5%B#$#!K3&))c! T&D!•739,!4\++F! H4WI!! J&'#$6,! jF! -Fv! (/$7@@2/7,! PF! PF! 3.'$D" E'06*:'*.D" O)9D! AKKU,!A?,!U[VYZF! H4YI!! `E&38&3A2,!JFv!(/%'/&**/,!MFCKFv!a#'@9R,!jFv!"#*2/&%,!PF! =$! E*.)0(.-6" 1'-I$*%&'0%v! K#*3/$7),! (F! KF,! M$)73A&,! GF,! L6)Fv! L')&E/&3! M@#6&B/@! K3&))c! MB)*&36#B•v! :7)*7$,! 4\\]v!>>!+\[V+4WF! !

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Enhanced Selectivity by Passivation: Molecular ... - ACS Publications

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