IDENTIFICATION AND CHEMICAL ASSAY OF NOREPINEPHRINE IN BRAIN AND OTHER TISSUES"* " A s i m p l e , r a p i d f l u o r o m e t r i c m e t h o d for t h e e s t i m a t i o n of : a t e c h o ! a m i n é s in b r a i n a n d t i s s u e s is d e s c r i b e d .
other
T h e p r o c e d u r e i n v o l v e s e x t r a c t i o n of c a t e c h o l a m i n e s f r o m t i s s u e h o m o g e n a t e s i n t o b u t a n o l . After returning, t h e a m i n e s to a n a q u e o u s p h a s e , t h e y a r e oxi i i i e d to form fluorescent d e r i v a t i v e s w h i c h a r e m e a s u r e d in a s p e c t r o f l w r o m e t e r . "
A c t i v a t i o n s p e e d a (left) a n d f l u o r e s c e n c e s p e c t r a (right) of v a r i o u > c a t e c h o l s a n d r a b b i t b r a i n e x t r a c t s . To o b t a i n the a c t i v a t i o n s p e c t r a , t h e f l u o r e s c e n c e m o n o c h r o m a t o r of a F a r r a n d R e c o r d i n g S p e c t r o f l u o r o m e t e r w a s s>t a t 5 2 0 ι η μ a n d t h e s p e c t r a from t h e a c t i v a t i n g r r o n o c h r o m a t o r w e r e s c a n n e d . To o b tain the fluorescence spectra, the activating mono c h r o m a t o r w a s ;«t a t 4 0 0 ιτιμ a n d t h e s p e c t r a f r o m t h e fluorescence· m o n o c h r o m a t o r w e r e s c a n n e d . %Ref: Parkhvist A . S h o r e and Jacqueline 5. O/in, J o u r n a l of Phorm. and f x p e r i m . T h e r a p e u t i c s , Vo. 1 2 2 , N o . 3 ,
FARRAND® SPECTROFLUOROMETEk Spectrofluorometric assay has distinct advantages over physical methods using light absorption. Fluorescent methods, where applicable^ are far more discrim inating than either Colorimetric or Spectrophotdmetric ai,say and yield a sensitivity at least two orders of magnitude greater than Spectrophotometry.
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