Author’s Accepted Manuscript Effect of thermal manipulation during embryogenesis on the promoter methylation and expression of myogenesis-related genes in duck skeletal muscle Yushi Wang, Xiping Yan, Hehe Liu, Shenqiang Hu, Jiwei Hu, Liang Li, Jiwen Wang www.elsevier.com/locate/jtherbio
PII: DOI: Reference:
S0306-4565(18)30110-4 https://doi.org/10.1016/j.jtherbio.2018.12.023 TB2243
To appear in: Journal of Thermal Biology Received date: 17 March 2018 Revised date: 22 December 2018 Accepted date: 24 December 2018 Cite this article as: Yushi Wang, Xiping Yan, Hehe Liu, Shenqiang Hu, Jiwei Hu, Liang Li and Jiwen Wang, Effect of thermal manipulation during embryogenesis on the promoter methylation and expression of myogenesisrelated genes in duck skeletal muscle, Journal of Thermal Biology, https://doi.org/10.1016/j.jtherbio.2018.12.023 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Effect of thermal manipulation during embryogenesis on the promoter methylation and expression of myogenesis-related genes in duck skeletal muscle Yushi Wanga,1, Xiping Yanb,1, Hehe Liua, Shenqiang Hua, Jiwei Hua, Liang Lia, Jiwen Wanga,* a
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan
Province, Sichuan Agricultural University, Chengdu 611130, PR China b
Agriculture Bureau of Ya'an, Ya'an 625000, PR China
1
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Abstract
Avian embryos are an ideal system to investigate the effect of incubation temperature on embryonic development, but the characteristics and mechanisms of temperature effects on poultry embryonic myogenesis are unclear. In this study, we investigated the effect of increasing the incubation temperature by 1°C on the expression of nine myogenesis-related genes in ducks and then explored the correlation between the alteration of promoter methylation and the expression of two of the nine genes under thermal manipulation (TM).
1
These authors contributed equally to this work
The qRT-PCR results showed that TM during embryonic days (ED) 1-10 promoted (P
