Genetically Encoded Multispectral Labeling of ... - ACS Publications

Jan 27, 2017 - original haloalkyl reagent B8 and have confirmed its identity by ... expressing a HaloTag fusion; these data are added to the corrected...
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Addition/Correction pubs.acs.org/JACS

Correction to “Genetically Encoded Multispectral Labeling of Proteins with Polyfluorophores on a DNA Backbone” Vijay Singh, Shenliang Wang, Ke Min Chan, Spencer A. Clark, and Eric T. Kool* J. Am. Chem. Soc. 2013, 135, 6184−6191. DOI: 10.1021/ja4004393 S Supporting Information *

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n internal review of our NMR data in the published Supporting Information (SI) file revealed that one of the authors (V.S.) had digitally removed peaks of impurities and solvents from some of the spectra. To correct this, we now provide a new version of the SI file with the unaltered spectra. Two new authors (K.M.C., S.A.C.) have recharacterized the original haloalkyl reagent B8 and have confirmed its identity by NMR and mass spectrometry. In addition, we have confirmed the identity of one of the original dyes by MALDI-TOF mass spectrometry, and used it successfully to label bacteria expressing a HaloTag fusion; these data are added to the corrected SI file. We stand by the conclusions of the article, and we regret the publication of the altered characterization data. We also correct the author list to include the scientists (K.M.C. and S.A.C.) who worked to independently check the data and conclusions. The new author list should read as follows: Vijay Singh, Shenliang Wang, Ke Min Chan, Spencer A. Clark, and Eric T. Kool*



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S Supporting Information *

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.6b11085. Experimental details, synthetic procedures, protein expression and labeling procedures, and NMR spectral data for new compounds (corrected) (PDF)

© XXXX American Chemical Society

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DOI: 10.1021/jacs.6b11085 J. Am. Chem. Soc. XXXX, XXX, XXX−XXX