Hydrophilic Chromatographic Determination of ... - ACS Publications

May 17, 2007 - BRYAN SAUNDERS , VITOR DE SALLES PAINELLI , LUANA FARIAS DE OLIVEIRA , VINICIUS DA EIRA SILVA , RAFAEL PIRES DA SILVA , LUIZ RIANI , MA...
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4664

J. Agric. Food Chem. 2007, 55, 4664−4669

Hydrophilic Chromatographic Determination of Carnosine, Anserine, Balenine, Creatine, and Creatinine LETICIA MORA, MIGUEL ANGEL SENTANDREU,*

AND

FIDEL TOLDRAÄ

Instituto de Agroquı´mica y Tecnologı´a de Alimentos (CSIC), P.O. Box 73, 46100 Burjassot, Valencia, Spain

A new HPLC procedure based on hydrophilic interaction chromatography (HILIC) has been developed for the simultaneous determination of carnosine, anserine, balenine, creatine, and creatinine in meat. This is the first time that HILIC has been directly applied to the study of meat components, having the advantage of not requiring complex cleanup and/or sample derivatization procedures. The chromatographic separation has been developed using a silica column (4.6 × 150 mm, 3 µm), and the proposed methodology is simple, reliable, and fast (