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Bioactive Constituents, Metabolites, and Functions
Fucoxanthin induces apoptosis in human glioma cells by triggering ROSmediated oxidative damage and regulating MAPKs and PI3K/AKT pathways Hua-lian Wu, Xiao-yan Fu, Wen-qiang Cao, Wen-zhou Xiang, Ya-jun Hou, Jin-kui Ma, Ying Wang, and Cundong Fan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b07126 • Publication Date (Web): 28 Jan 2019 Downloaded from http://pubs.acs.org on January 30, 2019
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Journal of Agricultural and Food Chemistry
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Fucoxanthin induces apoptosis in human glioma cells by
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triggering ROS-mediated oxidative damage and regulating
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MAPKs and PI3K/AKT pathways
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Hua-lian Wu1‡, Xiao-yan Fu2‡, Wen-qiang Cao3, Wen-zhou Xiang1, Ya-jun Hou2,
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Jin-kui Ma4, Ying Wang2*, Cun-dong Fan2*
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1
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Key Laboratory of Marine Materia Medica (LMMM-GD), South China Sea Institute
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of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
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Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong
Key Lab of Cerebral Microcirculation in Universities of Shandong, Taishan Medical
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University, Taian, Shandong, 271000, China
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3
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Guangdong, 519000, China
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4
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526061, China
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‡ Authors contributed equally to the work.
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* Corresponding authors：
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Cun-dong Fan, Key Lab of Cerebral Microcirculation in Universities of Shandong,
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Taishan Medical University, Taian, Shandong, 271000, China, Tel: +86-538-6230027;
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Fax: +86-538-6230027; Email:
[email protected] 20
Ying Wang, Key Lab of Cerebral Microcirculation in Universities of Shandong,
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Taishan Medical University, Taian, Shandong, 271000, China, Tel: +86-538-6230030;
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Fax: +86-538-6230030; Email:
[email protected] Zhuhai Hopegenes Medical & Phamaceutical Institute, Hengqin New Area, Zhuhai,
School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT Fucoxanthin a natural carotenoid derived from algae exhibits novel anticancer
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potential. However, fucoxanthin with high purity was hard to prepare and the
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anticancer mechanism remains elusive. In the present study, fucoxanthin with high
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purity was prepared and purified from marine micralgae Nitzschia sp. by silica gel
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column chromatography (SGCC), and the underlying mechanism against human
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giloma cells was evaluated. The results showed that fucoxanthin time- and
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dose-dependently inhibited U251 human glioma cells growth by induction of
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apoptosis (64.4±4.8, P
