Technical Note pubs.acs.org/ac
Integrated Microscale Analysis System for Targeted Liquid Chromatography Mass Spectrometry Proteomics on Limited Amounts of Enriched Cell Populations Jeffrey G. Martin,* Tomas Rejtar, and Stephen A. Martin Novartis Institutes for Biomedical Research, Cambridge, Massachusetts 02139, United States S Supporting Information *
ABSTRACT: Limited samples, such as those that are in vivo sourced via biopsy, are closely representative of biological systems and contain valuable information for drug discovery. However, these precious samples are often heterogeneous and require cellular prefractionation prior to proteomic analysis to isolate specific subpopulations of interest. Enriched cells from in vivo samples are often very limited (
