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Isoprene-Derived Organosulfates: Vibrational Mode Analysis by Raman Spectroscopy, Acidity-Dependent Spectral Modes, and Observation in Individual Atmospheric Particles Amy Lynne Bondy, Rebecca Lynn Craig, Zhenfa Zhang, Avram Gold, Jason Douglas Surratt, and Andrew P Ault J. Phys. Chem. A, Just Accepted Manuscript • DOI: 10.1021/acs.jpca.7b10587 • Publication Date (Web): 08 Dec 2017 Downloaded from http://pubs.acs.org on December 8, 2017

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The Journal of Physical Chemistry

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Isoprene-Derived Organosulfates: Vibrational Mode

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Analysis by Raman Spectroscopy, Acidity-

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Dependent Spectral Modes, and Observation in

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Individual Atmospheric Particles

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Amy L. Bondy, 1 Rebecca L. Craig, 1 Zhenfa Zhang,2 Avram Gold,2 Jason D. Surratt,2 Andrew P.

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Ault 1,3*

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1

Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109

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2

Department of Environmental Sciences and Engineering, Gillings School of Global Public

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Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599

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3

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48109

12 13 14

*corresponding author’s e-mail: [email protected]

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Journal of Physical Chemistry A

Department of Environmental Health Sciences, University of Michigan, Ann Arbor, Michigan,

ACS Paragon Plus Environment

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The Journal of Physical Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

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Abstract

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Isoprene, the most abundant biogenic volatile organic compound (BVOC) in the

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atmosphere, and its low-volatility oxidation products lead to secondary organic aerosol (SOA)

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formation. Isoprene-derived organosulfates formed from reactions of isoprene oxidation products

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with sulfate in the particle phase are a significant component of SOA and can hydrolyze forming

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polyols. Despite characterization by mass spectrometry, their basic structural and spectroscopic

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properties remain poorly understood. Herein, Raman microspectroscopy and density functional

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theory (DFT) calculations (CAM-B3LYP level of theory) were combined to analyze vibrational

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modes of key organosulfates, 3-methyltetrol sulfate esters and 2-methylglyceric acid sulfate

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esters (both racemic mixtures of two isomers), and hydrolysis products, 2-methyltetrols and 2-

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methylglyceric acid. Two intense vibrational modes were identified, ν(RO-SO3) (846±4 cm–1)

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and vs(SO3) (1065±2 cm–1), along with a lower intensity δ(SO3) mode (586±2 cm–1). For 2-

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methylglyceric acid and its sulfate esters, deprotonation of the carboxylic acid at pH values

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above the pKa decreased the carbonyl stretch frequency (1724 cm–1), while carboxylate modes

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grew in for νs(COO–) and νa(COO–) at 1413 and 1594 cm–1, respectively. The ν(RO-SO3) and

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vs(SO3) modes were observed in individual atmospheric particles and can be used in future

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studies of complex SOA mixtures to distinguish organosulfates from inorganic sulfate or

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hydrolysis products.

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Introduction

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Isoprene is a biogenic volatile organic compound (BVOC) emitted by broadleaf trees and

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is the largest global emission of all non-methane VOCs (~600 Tg y–1).1 Atmospheric oxidation

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of isoprene leads to lower volatility products that partition to the particle phase, forming

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secondary organic aerosol (SOA),2-5 which has been estimated to constitute 30% to 50% of the

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global SOA budget.4 SOA-containing particles impact climate by scattering and absorbing solar

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radiation or acting as cloud condensation nuclei (CCN).6 Recent work has also shown that

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exposure of human lung cells to atmospherically relevant isoprene-derived SOA induces

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oxidative stress.7-9 Furthermore, chronic obstructive pulmonary disease (COPD), which can

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worsen due to oxidative stress in the human respiratory system,10-11 is higher in the southeastern

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United States where isoprene-derived SOA contributes large mass fractions (up to 40%) of

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submicron organic aerosol.12-14 Therefore, understanding the formation and evolution of

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isoprene-derived SOA is important for understanding the overall impact of aerosols on climate

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and health.

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Organosulfates are a major class of compounds in SOA, estimated to contribute 5-10% of

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the total organic aerosol mass over the continental US.15 Due to their high polarity and water

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solubility, these hygroscopic compounds could enhance the CCN activity of organic aerosol.16

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Isoprene-derived organosulfates formed from reactions of sulfate with isoprene oxidation

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products in the particle phase are often reported as the most abundant organosulfates in ambient

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aerosols,17-20 with mass concentrations of up to 8% of organic matter.21-24 The isoprene-derived

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organosulfates detected in ambient aerosol formed under low-NOx conditions are the

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diastereomeric methyltetrol sulfate ester racemates,17-23, 25-29 while under high-NOx conditions,

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racemic 2-methylglyceric acid sulfate ester is also detected.17-18, 20-22, 25, 29 A simplified scheme of


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reactions leading to the formation of isoprene-derived organosulfates under low- and high-NOx

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conditions is shown in Figure 1. The sulfate esters form via a nucleophilic oxirane ring-opening

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reaction by sulfate. Sulfate attacks δ-isoprene epoxydiol (δ-IEPOX), an isomer of IEPOX

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formed in significant proportion,30 under low-NOx conditions at the primary carbon (C1) to yield

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racemic 3-methylerythritol sulfate ester ((2S,3R)/(2R,3S)-2,3,4-trihydroxy-3-methylbutyl sulfate)

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and racemic 3-methylthreitol sulfate ester ((2R,3R)/(2S,3S)-2,3,4-trihydroxy-3-methylbutyl

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sulfate).31-33 Two high NOx isoprene-oxidation products, methacrylic acid epoxide (MAE; 2-

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methyoxirane-2-carboxylic acid) and hydroxymethyl-methyl-α-lactone (HMML), yield racemic

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2-methylglyceric acid sulfate ester (2-carboxyl-2-hydroxylpropyl sulfate). Methyltetrol sulfate

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esters have been observed in more than 65% of particles in the southeastern US and are among

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the most abundant individual organic compounds in atmospheric aerosol.17, 19 Although previous

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studies in China and the southeastern United States showed that 2-methylglyceric acid sulfate

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ester is not as abundant in ambient aerosol as the methyltetrol sulfate esters, it nevertheless

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accounted for ~0.5% of organic matter by mass.21-22 Isoprene-derived organosulfates have long

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atmospheric lifetimes and thus are substantial contributors to ambient organic aerosol.31 The

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sulfate esters can undergo hydrolysis within particles (hydrolysis lifetime, 60 to 460 h,

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depending on particle acidity),31, 34 leading to the formation of the diastereomeric 2-methyltetrol

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racemates (erythritol and threitol) and racemic 2-methylglyceric acid (2,3-dihydroxy-2-

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methylpropanoic acid).22, 25, 31-32, 34-36 The prevalence of these organosulfates and their hydrolysis

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products as well as their continuing chemistry in the aerosol phase, has recently elicited

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considerable research interest.5, 20, 27, 29, 35, 37-39


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Figure 1. Scheme leading to the formation of isoprene-derived SOA compounds: 3-methyltetrol sulfate esters, 2-methyltetrols, 2-methylglyceric acid sulfate ester, and 2-methylglyceric acid. Both the high- and low-NOx pathways are shown.22, 32, 36 For simplicity, only one isomer of each respective compound is shown. 2-Methylglyceric acid, 2-methylglyceric acid sulfate ester, 2methyltetrols, and the 3-methyltetrol sulfate esters are present in the particle phase, while the epoxides are in the gas phase.

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Currently, identification and quantitation of isoprene-derived organosulfates in individual

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aerosol particles has been limited due to the complexity of SOA particles (thousands of species


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are often present in attoliter volumes), instrumental difficulties in differentiating organosulfates

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from inorganic sulfate, and a lack of authentic standards. Most methods currently used to detect

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organosulfate species have been offline mass spectrometry-focused, relying on filter extractions

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followed by ultra-performance liquid chromatography/electrospray ionization high-resolution

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quadrupole time-of-flight mass spectrometry (UPLC/ESI-HR-QTOFMS).20-22,

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Online analysis has been attempted by Farmer et al., who used an online aerosol mass

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spectrometer (AMS) to analyze an isoprene organosulfate surrogate standard, though due to

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extensive fragmentation, this compound could not be differentiated from inorganic sulfate in

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ambient aerosol.45 Some spectroscopic analysis has been done on organosulfates using Fourier

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transform infrared spectroscopy,46-50 but identification has focused on a single lower frequency

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vibration (C-S of methane sulfonic acid at 876 cm–1), rather than the entire fingerprint region,

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making interpretation challenging. Although bulk methods are typically used to detect and

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quantify organosulfates, they provide information limited to average aerosol composition and do

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not provide information concerning the abundance of organosulfate-containing particles.

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24-29, 32, 36, 40-44

Single particle mass spectrometry methods have been used to provide information

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regarding the mixing state of organosulfates.

Hatch et al. detected isoprene-derived

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organosulfates using aerosol time-of-flight mass spectrometry (ATOFMS), and observed high

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fractions during two field studies in Atlanta, Georgia.17-18 Froyd et al. detected significant levels

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of isoprene-derived organosulfates in the free troposphere by particle ablation laser mass

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spectrometry (PALMS).19 These studies revealed that isoprene-derived organosulfates not only

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account for a sizeable fraction of organic aerosol mass, but also are ubiquitous, present in more

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than 70% of aerosols over the continental US.17, 19 However, issues such as fragmentation and


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shot-to-shot variability for laser desorption/ionization in both the ATOFMS and PALMS17, 19, 23

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make quantifying organosulfates particularly challenging.

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In contrast to bulk analysis and single particle mass spectrometry methods, Raman

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microspectroscopy is a nondestructive technique that can be used to analyze the vibrational

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modes of functional groups within individual particles.51-66 In addition to identifying vibrational

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modes, Raman microspectroscopy is sensitive to changes in bonding and molecular environment,

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such as aqueous versus solid particles67 or acidity-dependent protonation states.55,

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Raman scattering, unlike mass spectrometry, is not dependent on particle ionization and

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fragmentation patterns,19,

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such as inorganic sulfate and organosulfates, since each class exhibits unique vibrational modes.

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Previous Raman studies of ambient SOA identified nitrates, sulfates, carbonates, alcohols/water,

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silicates and aluminosilicates, soot, hydrocarbons, and humic like substances within individual

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particles.53, 56-57, 59-60 However, despite the rich vibrational spectra that Raman can provide, the

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complexity of ambient SOA makes unambiguous identification of specific constituents

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challenging. In order to identify isoprene-derived organosulfates within ambient SOA particles

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using Raman spectroscopy, a thorough analysis of their vibrational modes is therefore necessary.

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Herein,

the

45

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Since

it can be used to differentiate classes of compounds within SOA,

Raman

vibrational

spectra

of

the

atmospherically-relevant

3-

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methylerythritol/3-methylthreitol sulfate ester mixture derived from δ-IEPOX and 2-

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methylglyceric acid sulfate ester derived from MAE and HMML are recorded and observed band

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frequencies are compared to calculated frequencies. Raman spectra of the organosulfate

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hydrolysis products, 2-methyltetrols and 2-methylglyceric acid, were examined to assist in the

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identification of the organosulfate-related modes. Density functional theory (DFT), in

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conjunction with published frequencies of small-molecule organosulfates, was used to predict


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optimized structures of the isoprene-derived organosulfates and to assign vibrational modes in

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experimental Raman spectra. Acidity-dependent shifts of key modes were identified.

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Furthermore, key organosulfate-related Raman modes were observed in ambient atmospheric

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aerosol particles collected from the southeast United States. This study identifies signature

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Raman vibrational modes for isoprene-derived organosulfates that can be used to identify these

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compounds in chamber studies and ambient particles.

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Experimental Methods

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Reagents. 2-Methylglyceric acid, racemic 2-methylglyceric acid sulfate ester potassium

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salt (81%), a diastereomeric mixture of racemic 2-methyltetrols (racemic 2-methylerythritol and

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racemic 2-methylthreitol), and sodium salts of the 3-methyltetrol sulfate esters (86%) (racemic 3-

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methylerythritol sulfate ester and racemic 3-methylthreitol sulfate ester) were synthesized by the

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Surratt group and used without further purification.24,

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methyltetrol sulfate esters and 2-methyltetrols are given in the Supporting Information (SI). 2-

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Methylglyceric acid was synthesized by a published method.69 2-Methylglyceric acid sulfate

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ester potassium salt was synthesized with a slight modification of the published method41 using

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methylglyceric acid as the starting material. Trace quantities of inorganic sulfate are present in

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the 3-methyltetrol and 2-methylglyceric acid sulfate esters. Target structures were verified by

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proton nuclear magnetic resonance spectroscopy (1H NMR), Fourier transform infrared

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spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDX) (SI, Figures S1-S8).

41, 68-69

Synthetic details for the 3-

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Raman Microspectroscopy. Aqueous 0.05 M solutions of 2-methylglyceric acid, 2-

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methylglyceric acid sulfate ester, 2-methyltetrols, and 3-methyltetrol sulfate esters were prepared

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by dissolution in 18.3 MΩ Milli-Q water. For Raman analysis, 2 µL droplets (~1 mm diameter)


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were deposited onto quartz substrates (Ted Pella). The relatively dilute solutions with low ionic

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strengths used in this work most likely rules out non‐ideal solution effects on the Raman spectra.

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Additionally, aerosol particles were generated by nebulizing aqueous 0.05 M solutions of 2-

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methylglyceric acid sulfate ester and 3-methyltetrol sulfate esters onto quartz using a concentric

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glass nebulizer (TR-30-A1, Meinhard). Particles from aerosolized compounds, with projected

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area diameters ranging from ~3.5 to 9 µm, produced identical, but lower intensity Raman spectra

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as large droplets of the aqueous solutions, therefore only Raman spectra of the organosulfate

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solutions are shown in the subsequent analysis. See SI for Raman spectra of aerosolized

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compounds and pure crystals/liquids (Figures S9-S12).

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The standards were probed using a Raman microspectrometer (LabRAM HR Evolution,

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HORIBA, Ltc.) at ambient temperature and relative humidity. The Raman spectrometer was

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coupled with a confocal optical microscope (100x long working distance Olympus objective, 0.9

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numerical aperture) and equipped with a Nd:YAG laser source (50 mW, 532 nm) operated with a

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neutral density (ND) filter at 100% and CCD detector. The 1800 g/mm diffraction grating

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yielded a spectral resolution of ~0.7 cm–1. The instrument was calibrated daily using a silicon

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wafer standard against the Stokes Raman signal of pure Si at 520 cm–1. Spectra were collected

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with three accumulations at 60-second acquisition times for the following spectral ranges: 500-

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4000 cm–1 (2-methylglyceric acid sulfate ester and 2-methyltetrols), 500-2200 cm–1 and 2200-

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4000 cm–1 (2-methylglyceric acid), 500-1800 cm–1 and 2500-4000 (3-methyltetrol sulfate esters).

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For 2-methylglyceric acid and the 3-methyltetrol sulfate esters, the lower frequency ranges (500-

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2200 cm–1 and 500-1800 cm–1, respectively) and the higher frequency range (2500-4000 cm–1)

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were collected separately using the same number of accumulations and acquisition time

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described previously to minimize water evaporation.


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To explore the nature of the carboxylic acid vibrational mode and identify the cause of

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spectral variation between 2-methylglyceric acid sulfate ester and 2-methylglyceric acid, the

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effect of pH was studied. Assuming a similar pKa for 2-methylglyceric acid compared to glyceric

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acid (pKa glyceric acid ~3.5), the pH of an aqueous solution of 2-methylglyceric acid was

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adjusted from 1.3 to approximately 1.8, 3, and 10 through the addition of NaOH, measured using

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pH paper. Raman spectra were collected of the aqueous solutions of 2-methylglyceric acid at

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each pH from 500 to 4000 cm–1 using three accumulations, 10-second acquisition times, and a

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600 g/mm diffraction grating with a spectral resolution of ~1.7 cm–1.

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Density Functional Theory Calculations. Geometry optimization, Raman shift, and

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Raman scattering activity calculations were performed using Gaussian 09W.70 Initial geometry

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optimizations were performed using DFT with the CAM-B3LYP functional and 3-21G basis set.

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An additional geometry optimization was performed and Raman vibrational mode frequencies

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were calculated at the DFT CAM-B3LYP level of theory with the 6-311 ++ G(2d,p) basis set,

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using an ultrafine pruned grid and tight optimization criteria. All calculations are unscaled and

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run using water as a solvent. Calculated Raman frequencies and activity were used, in

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conjunction with literature,71-77 to assign vibrational modes to the experimental Raman spectra

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collected for each compound. The DFT calculations for 2-methylglyceric acid sulfate ester, 3-

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methylerythritol sulfate ester, and 3-methylthreitol sulfate ester were performed with a -1 charge

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on the compounds. Since the DFT calculated frequencies for the sulfate functional group-related

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modes deviate from literature frequencies, multiple functionals and basis sets were tested for

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methyl sulfate, a model compound (Table S1), and CAM-B3LYP was selected for the following

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analysis as a compromise between accuracy and calculation expense.


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Ambient Aerosol Collection. Ambient particles from the Southern Oxidant and Aerosol

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Study (SOAS) during the summer of 2013 were analyzed for isoprene-derived organosulfate

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Raman signatures. Description of the site and particle collection is provided elsewhere.78-80

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Briefly, particles were impacted on quartz substrates (Ted Pella Inc.) using a micro-orifice

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uniform deposit impactor (MOUDI, Model 110, MSP Corp.) in Centreville, AL, a rural, forested

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region near Talladega National Forest with high isoprene emissions. After collection, all

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substrates were sealed and stored at -22 °C prior to analysis. Individual particles were analyzed

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using computer controlled-Raman microspectroscopy (CC-Raman), according to the method

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described previously by Craig et al.54

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Results and Discussion

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To identify Raman signatures for isoprene-derived organosulfates, differences between

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the experimental spectra of the organosulfates and their hydrolysis products were noted. Figure 2

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shows experimental Raman spectra of the organosulfates and their hydrolysis products overlaid

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with intensities normalized to the modes at 1460 cm–1 and 2950 cm–1 (the most intense modes in

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the low- and high-frequency regions, respectively, excluding possible sulfate modes). Distinct

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modes present in the spectra of both organosulfates at ~1065 cm–1 and ~850 cm–1, assigned to the

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organosulfate functional group, are highlighted. Aside from the sulfate modes, key spectral

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differences include a very intense inorganic sulfate mode (from synthesis or hydrolysis) present

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in the spectrum of the 3-methyltetrol sulfate ester mixture at 982 cm–1, and differences in the

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carbonyl stretching region (1400-1750 cm–1) for 2-methylglyceric acid sulfate ester and 2-

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methylglyceric acid which will be explored in more detail below.


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Figure 2. Experimental Raman spectra of a) the 3-methyltetrol sulfate esters overlaid with 2methyltetrols, and b) 2-methylglyceric acid sulfate ester overlaid with 2-methylglyceric acid.

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To correlate the spectral differences observed in Figure 2 with the presence of the

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organosulfate functional group, quantum chemical calculations were performed for the 3-

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methyltetrol sulfate esters, 2-methyltetrols, 2-methylglyceric acid sulfate ester, and 2-

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methylglyceric acid. Figure 3a shows experimental Raman modes within the fingerprint region

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(500-1500 cm–1) and at higher frequencies (2700-3500 cm–1) for the mixture of diastereomeric 3-

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methyltetrol sulfate esters in an aqueous solution. Using DFT, the Raman activity (Figure 3b)

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was calculated for the minimum-energy geometry of each diastereomer (Figure 3c and 3d). The

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optimal geometries, with the terminal (C4) hydroxyl group twisted toward the sulfate group,

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were used in the subsequent analysis, although the energies were not substantially different for

233

other conformers (difference

Isoprene-Derived Organosulfates: Vibrational ... - ACS Publications

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