Kinetics of the Formalin Inactivation of Po1iov;riis
It, is siiimn tlmt tile non-first-order course of tlie Eoriii:ilin intictivation (If poliiJvirus is consistent with t i l e liinetic requirements of a mechanism of the type, N,, nN nu. In this forrnulation, Si, represents :L noninfectious n-meric form of the virus, N is tile infectious native form of the virus, :tiid I > is an antigenic but noninfectious species. T h e method used for testing tlie applicability of this riiecli:inism is discussed in some detail. Several respects in wV2iic.h the postulated meclistnism is related to previous propi)s:ils :ire :ilso esxmined, especi:rily the possible role of :L snperirnposed thernid denaturation. .-f
It is customary' to describe t'he time course of the denaturation of proteins, including \ - i r ~ i s e sby , ~ ~a ~semiJ. I). Eiickson, 189 (1914). (L'j 11.-1.Lauffer, J . .Im. Ciiem. ,Sot., 66, 17031 Il91:j). (:L R . ,A. c'. Foster, F. H. Johnson, a n d \-. Ii. l I i l i e r , J . Oen. I'hysiol.. 33, I (1 !149). (1'1 JI. Neurath. ,I. 1'. Grernstrin, I:. T\-, I'ritnani, and
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