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Allium cepa L. response to sodium selenite (Se(IV)) studied in plant roots by LC-MS-based proteomic approach Jakub Karasinski, Kazimierz Wrobel, Alma Rosa Corrales Escobosa, Anna Konopka, Ewa Bulska, and Katarzyna Wrobel J. Agric. Food Chem., Just Accepted Manuscript • Publication Date (Web): 03 May 2017 Downloaded from http://pubs.acs.org on May 4, 2017
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Allium cepa L. response to sodium selenite (Se(IV)) studied in plant roots by
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LC-MS-based proteomic approach
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Jakub Karasinskib, Kazimierz Wrobela, Alma Rosa Corrales Escobosaa, Anna Konopkab,
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Ewa Bulskab, Katarzyna Wrobela*
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a
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Mexico
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b
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Żwirki i Wigury 101, 02-089 Warsaw, Poland
Department of Chemistry, University of Guanajuato, L de Retana N°5, 36000 Guanajuato,
Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw,
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* Corresponding author (
[email protected]), Phone (+52) 473 7327555, Fax (+52) 473 7326252. 1 ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Liquid chromatography-high resolution mass spectrometry was used for the first time to
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investigate the impact of Se(IV) (10 mgSe L-1 as sodium selenite) on Allium cepa L. root
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proteome. Using MaxQuant platform, more than 600 proteins were found; 42 were identified
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based on at least 2 razor+unique peptides, score > 25 and were found to be differentially
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expressed in the exposed versus control roots with t-test difference > +/- 0.70 (p
