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Low Focal Adhesion Signaling Promotes Ground State Pluripotency of Mouse Embryonic Stem Cells Sara Taleahmad, Mehdi Mirzaei, Azam Samadian, Seyedeh Nafiseh Hassani, Paul A. Haynes, Ghasem Hosseini Salekdeh, and Hossein Baharvand J. Proteome Res., Just Accepted Manuscript • DOI: 10.1021/acs.jproteome.7b00322 • Publication Date (Web): 29 Aug 2017 Downloaded from http://pubs.acs.org on August 30, 2017
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Journal of Proteome Research
Low Focal Adhesion Signaling Promotes Ground State Pluripotency of Mouse Embryonic Stem Cells Sara Taleahmad1, Mehdi Mirzaei2,3,4, Azam Samadian5, Seyedeh-Nafiseh Hassani5, Paul A. Haynes2, Ghasem Hosseini Salekdeh1,6,*, Hossein Baharvand5,7,* 1. Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. 2. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia. 3. Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia 4. Australian Proteome Analysis Facility, Macquarie University, Sydney, Australia 5. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. 6. Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran. 7. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.
*Corresponding authors: Hossein Baharvand, Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box: 16635-148, Tehran, Iran. Postal Code: 1665659911, Tel: +98 21 22306485, Fax: +98 21 23562507, Email:
[email protected] or Ghasem Hosseini Salekdeh, Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Tel: +98 21 22306485, Fax: +98 21 23562507, Email:
[email protected] 1 Environment ACS Paragon Plus
Journal of Proteome Research
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Abstract Mouse embryonic stem cells (mESCs) can be maintained in a pluripotent state when cultured with 2 inhibitors (2i) of extracellular signal-regulated kinase (MEK) and glycogen synthase kinase-3 (GSK3), and Royan 2 inhibitors (R2i) of FGF4 and TGFβ. The molecular mechanisms that control ESC self-renewal and pluripotency is more important for translating stem cell technologies to clinical applications. In this study, we used the shotgun proteomics technique to compare the proteome of the ground state condition (R2i- and 2i-grown cells) to serum. Out of 1749 proteins identified, 171 proteins were differentially expressed (p
