Anal. Chem. 2007, 79, 9310-9314
Luminescence Lifetime Standards for the Nanosecond to Microsecond Range and Oxygen Quenching of Ruthenium(II) Complexes Kaleem J. Morris,† Michael S. Roach,† Wenying Xu,† J. N. Demas,*,† and B. A. DeGraff‡
Chemistry Department, University of Virginia, Charlottesville, Virginia 22904, and Chemistry Department, James Madison University, Harrisonburg, Virginia 22807
A rapid and reproducible method for determining the temperature dependence of luminescence lifetimes has been developed. With the use of this method, a set of standards for the excited-state lifetime oxygen quenching of several ruthenium(II) transition metal complexes was established. With the use of three solvents of different viscosities and two metal complexes with widely different lifetimes, an overlapping range of ca. 100 ns to 6 µs was obtained. The decays are pure single exponentials, which means that they can be used reliably with both phase and pulsed lifetime instruments. For a pure single-exponential decay, a properly operating phase shift instrument will give the same lifetime as a time domain instrument. With the use of a thermal deactivation model and a threeparameter temperature-dependent oxygen quenching constant, the lifetime temperature-dependent data was well fit by a simple six-parameter equation that covers the temperature range of 10-50 °C and oxygen pressures from 0 to 1 atm of oxygen with excellent precision (ca.
