Biochemistry 1993, 32, 3027-3037
3027
Mechanism of Calf Thymus DNA Primase: Slow Initiation, Rapid Polymerization, and Intelligent Terminationt Robert J. Sheaff and Robert D. Kuchta’ Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215 Received August 20, 1992; Revised Manuscript Received January 11, I993
The mechanism by which calf thymus DNA primase synthesizes R N A primers was examined. Primase first binds a single-stranded DNA template (KD