Metabolomics Analysis To Evaluate the Anti ... - ACS Publications

Jun 23, 2017 - Metabolomics Analysis To Evaluate the Anti-Inflammatory Effects of. Polyphenols: Glabridin Reversed Metabolism Change Caused by LPS...
1 downloads 0 Views 2MB Size
Subscriber access provided by EAST TENNESSEE STATE UNIV

Article

Metabolomics analysis to evaluate the antiinflammatory effects of polyphenols: glabridin reversed metabolism change caused by LPS in RAW 264.7 cells. Kaiqin Liu, FuWei Pi, Hongxia Zhang, Jian Ji, Shuang Xia, Fangchao Cui, Jiadi Sun, and Xiulan Sun J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b01692 • Publication Date (Web): 23 Jun 2017 Downloaded from http://pubs.acs.org on June 24, 2017

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 38

Journal of Agricultural and Food Chemistry

1

Metabolomics Analysis To Evaluate The

2

Anti-Inflammatory Effects Of Polyphenols: Glabridin

3

Reversed Metabolism Change Caused By LPS In

4

RAW 264.7 Cells.

5

Kaiqin Liua, Fuwei Pia, Hongxia Zhangb, Jian JIa, Shuang Xiaa, Fangchao Cuia,

6

Jiadi Suna, Xiulan Suna*

7

a

8

Technology, National Engineering Research Center for Functional Food,

9

Jiangnan University, Wuxi, Jiangsu 214122, PR China

*

School of Food Science and Technology, Laboratory of Food Science and

10 11

b

School of Foreign Studies, Shaanxi University of Technology, 723000, China

12 13 14 15 16 17 18

Corresponding author*: Xiulan Sun (E-mail: [email protected])

19

Address: Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,PR

20

China.

21

E-mail: [email protected]; Tel:+86-510-85912330

22

Fax: 85329015

23

running title: Metabolomics Analysis To Evaluate The Anti-Inflammatory Effects

24

1

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

Page 2 of 38

25

ABSTRACT:

26

Inflammation has been shown to play a critical role in the development of many

27

diseases. In this study, we used metabolomics to evaluate the inflammatory effect of

28

lipopolysaccharide (LPS) and anti-inflammatory effect of glabridin (GB, a polyphenol

29

from Glycurrhiza glabra L. roots) in RAW 264.7 cells. Multivariate statistical analysis

30

showed that compared with the LPS group, the metabolic profile of the GB group was

31

more similar to that of the control group. LPS impacted the amino acid, energy and

32

lipid metabolisms in RAW 264.7 cells and metabolic pathway analysis showed that

33

GB reversed some of those LPS impacts. Metabolomics analysis provided us a new

34

perspective to better understand the inflammatory response and the anti-inflammatory

35

effects of GB. Metabolic pathway analysis can be an effective tool to elucidate the

36

mechanism of inflammation and to potentially find new anti-inflammatory agents.

37

Keywords:

38

anti-inflammatory effect; polyphenols

cells

metabolomics;

metabolism

pathway

39 40 41 42 43 44 45 46 2

ACS Paragon Plus Environment

analysis;

LPS;

Page 3 of 38

Journal of Agricultural and Food Chemistry

47

1.Introduction

48

Inflammation is a normal protective response of the innate immune system to tissue

49

injury or detrimental external stimuli such as pathogens, allergens, and other irritants1.

50

Efficient control of inflammation is vital to protect the host from external stimuli.

51

Defective resolution of inflammation and ‘misreading’ of inflammatory signals

52

increases the risk of developing autoimmune and chronic inflammatory diseases.

53

During the inflammatory reaction, immune system cells can recognize foreign

54

pathogenic molecules, such as lipopolysaccharide (LPS), which is recognized by

55

Toll-like receptors (TLRs) or NOD-like receptors1. This process can lead to the

56

activation of major MAP kinase cascades and translocation of the regulator nuclear

57

factor-κB (NF-κB) , resulting in the increased expression of pro-inflammatory

58

cytokines, such as interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor α

59

(TNF-α), monocyte chemotactic factor 2(CCL-2)2. In addition, it will lead to

60

increased expression of nitric oxide (NO) synthase and thus excess NO accumulation.

61

Pro-inflammatory cytokines play a key role in some cardiovascular disease (CVD)

62

and chronic inflammatory diseases, such as insulin resistance3, diabetes4, 5,

63

atherosclerosis6 and depression2, 7.

64

Polyphenols are one of the most important group of natural antioxidants and

65

chemopreventive agents found in fruits, vegetables, grains, tea and essential oils8, 9.

66

Many investigations have repeatedly reported the anti-inflammatory functions of

67

different flavonoid molecules, such as suppressing inflammatory cytokines10,

68

modulating transcription factors and inflammation-related pathways, reducing 3

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

69

accumulation of NO or reactive oxygen species (ROS). Glabridin (GB) is a

70

species-specific isoflavan from Glycurrhiza glabra L. roots. It is reported that its

71

biological activities include antimicrobial, anti-inflammatory and cardiovascular

72

protective11. GB was found to attenuate LPS-induced production of inflammatory

73

mediators, including NO, TNF-α and IL-1β in THP-1 cells12, RAW 264.7 cells13 and

74

J774a.1 cells14. GB inhibited dendritic cell maturation by blocking NF-κB and MAPK

75

signalings15. Cao et al.16 have performed a pharmacokinetics of GB in

76

Sprague-Dawley rats. At 4.5 h after oral administration of 20 mg/kg GB, the peak

77

plasma level of GB up to 60.41 ng/mL (