ARTICLE pubs.acs.org/ac
Nanofluidic Devices with Two Pores in Series for Resistive-Pulse Sensing of Single Virus Capsids Zachary D. Harms,† Klaus B. Mogensen,‡ Pedro S. Nunes,‡ Kaimeng Zhou,† Brett W. Hildenbrand,† Indranil Mitra,† Zhenning Tan,§ Adam Zlotnick,§ J€org P. Kutter,‡ and Stephen C. Jacobson†,* †
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, United States Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby, Denmark § Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana 47405, United States ‡
ABSTRACT: We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing of hepatitis B virus (HBV) capsids. The nanochannel and two pores are patterned by electron beam lithography between two microchannels and etched by reactive ion etching. The two nanopores are 50-nm wide, 50-nm deep, and 40-nm long and are spaced 2.0-μm apart. The nanochannel that brackets the two pores is 20 wider (1 μm) to reduce the electrical resistance adjacent to the two pores and to ensure the current returns to its baseline value between resistive-pulse events. Average pulse amplitudes differ by
