1608
Chem. Res. Toxicol. 1998, 11, 1608
Additions and Corrections NO Release from NO Donors and Nitrovasodilators: Comparisons between Oxyhemoglobin and Potentiometric Assays [Volume 11, Number 12, December 1998, pp 1393-1397] JENNIFER D. ARTZ AND GREGORY R. J. THATCHER* Page 1394. In the last line of the caption of Figure 1, the concentration should be 3.8 µM. Page 1395. In Table 1, several concentrations should have had units of micromolar. See the table below. Table 1. Potentiometrically Determined Rate Constants (A) and Rates (B) for NO Release from Nitrovasodilators, Calibrated against Spectrophotometric Determinations for DEA/NO A
kNO (s-1)a
Tb (°C)
Bg
adjuvant
d[NO]/dt (nM/s)h
mediumi
DEA/NO DEA/NO NOC9/NO nPrONOc nPrONO nPrONOd ClCH2CH2ONOc tBuONOc tBuSNO2 SNAPe tBuSNOf
8.3 × 10-3 1.2 × 10-3 4.0 × 10-3 1.3 × 10-4 1.2 × 10-4 1.4 × 10-4 2.2 × 10-5 8.0 × 10-6 8.0 × 10-6 1.5 × 10-3 e 6.2 × 10-5
37 21 21 37 37 37 37 37 21 21 21
1 mM GTN 1 mM GTN 500 µM GTN 500 µM GTN 250 µM GTN 250 µM GTN 1 mM GTN 250 µM GT027 250 µM ClDN 500 µM GT002
1 mM FeIITPPj 50 mM TSA 1 mM TSA 500 µM TSA 1 mM Cys 500 µM TSA 500 µM TSA 1 mM TSA
9.2 ≈0k,l 37 ≈0l 61 9.1 ≈0l 51 15 3.1
21 °Cj 37 °C BP, 37 °C BP, 37 °C HP, 37 °C HP, 37 °C HP, 37 °C HP, 37 °C HP, 37 °C HP, 37 °C
a Determined from plots of observed rate vs concentration for at least three nitrovasodilator concentrations in triplicate. b Phosphate buffer at 37 °C, 100 mM, and pH 7.4 or at 21 °C, 50 mM, and pH 7.6, except DEA/NO, NOC9/NO (100 mM and pH 7.4). c [EDTA] ) 10 µM. d [Cys] ) 5 mM. e [Cys] ) 0.4 mM; spectrophotometric k(degradation) ) 2.0 × 10-3 s-1, and kNO(SNOG) is below detection limits. f Contains t-BuSH, and t-Bu-SS-t-Bu. g No NO was observed in HP from GT016 and TSA, GT017 and TSA, GT009 and TSA, GTN and DTT, and GTN and GSH. h Calibration by DEA/NO (kNO ) 2.2 × 10-3 in 100 mM phosphate buffer at pH 7.86 and 37 °C). i Phosphate buffer (100 mM) at pH 7.4, HP (human plasma), or BP (bovine plasma). j In 1:1 CH2Cl2/aqueous phosphate (pH 7.6 and 50 mM). k With or without SOD. l Below detection limits.
TX980470V 10.1021 Published on Web 12/21/1998
