Nontargeted Nucleotide Analysis Based on Benzoylhistamine

Mar 12, 2012 - Pharmaceutical Science and Barnett Institute, Northeastern University, ... La Jolla Institute for Allergy & Immunology, La Jolla, Calif...
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Nontargeted Nucleotide Analysis Based on Benzoylhistamine Labeling-MALDI-TOF/TOF-MS: Discovery of Putative 6-Oxo-Thymine in DNA Poguang Wang,† David Fisher,‡ Anjana Rao,§ and Roger W. Giese*,† †

Pharmaceutical Science and Barnett Institute, Northeastern University, Boston, Massachusetts 02115, United States Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, United States § La Jolla Institute for Allergy & Immunology, La Jolla, California 92307, United States ‡

ABSTRACT: A method for nontargeted analysis of modified nucleotides in DNA (and RNA) is reported based on labeling with benzoylhistamine (BH) followed by MALDI-MS. The method provides deoxynucleotide-specific detection, accurate measurement of molecular ions, high sensitivity, semiquantitation, and, to the extent studied to date, normalization of response within a factor of