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Occurrence and Inducibility of Cytochrome P450IIIA in Maternal and Fetal Rats during Prenatal Development? Janis E. Hulla and Mont R. Juchau* Department of Pharmacology, SJ-30, University of Washington School of Medicine, Seattle, Washington 981 95 Received November 4, 1988; Revised Manuscript Received January 17, 1989
ABSTRACT: The purpose of this study was to quantify cytochrome P450IIIA1 in fetal and maternal livers of uninduced and pregnenolone- 16a-carbonitrile (PCN) induced rats during the course of prenatal development. The activities and levels of P450IIIA in hepatic microsomes from maternal rats and fetuses a t 15-2 1 days of gestation were measured by triacetyloleandomycin (TAO) inhibited debenzylation of (benzy1oxy)phenoxazone and by immunoassay with defined antiserum specific for P450IIIA. P450IIIA was not detectable (
