EXTEND MOLECULAR CUTOFF LIMITS IN GEL FILTRATION
Sephadex
®G-100
G-200 "new 'highs for macromolecule separation' What they
tile
SEPHADEX
chemical stability, a n d upon swelling they p r o d u c e three dimensional networks devoid of ionic groups. P r e p a r e d in bead form, G - 2 0 0 a n d especially G - 1 0 0 offer very low flow resistance permitting the use of fine particles for good reso lution of separation.
G - 1 0 0 and G - 2 0 0
are like the already well-known SEPHADEX series G - 2 5 , G - 5 0 and G - 7 5 dextran gels, produced by cross-linking dcxtran chains with r a n d o m ether bonds between glucose residues in the polysaccharide chains. T h e y have good mechanical and
What they do The two new
SEPHADEX
Figure I shows a separation of pancreatic enzymes. Pow dered swine pancreas w a s extracted with a 0 . 0 5 M acetate buffer p H 5.3 containing 0.005 M calcium acetate. A 3 ml. sample was introduced in a 2 χ 37 c m . ( 1 1 7 m l . ) column packed with SEPHADEX G - 1 0 0 .
types
offer an i m p o r t a n t extension of the gel filtration m e t h o d for the fractionation of macromolecules. SEPHADEX G - 1 0 0 sep arates substances with molecular weights smaller than about 100,000. SEPHADEX G-200 separates substances with molec ular weights smaller than about 2 0 0 , 0 0 0 . A s with the other S E P H A D E X types, G - 1 0 0 a n d G - 2 0 0 act as molecular sieves. Molecules of larger dimension than the matrices of the par ticles do not penetrate the swollen gel. Fig. I a typical separation with S E P H A D E X G - 1 0 0
Fig. II a typical separation with S E P H A D E X G - 2 0 0 FRACTIONATION OF SERUM WITH SEPHADEX G-200
FRACTIONATION OF PANCREATIC ENZYMES WITH SEPHADEX G-100
Sample: 50 ml. swine serum. Column size: 1800 ml. (7.5x41 cm.).
Figure II shows a separation of serum proteins. A c o l u m n 7.5 χ 41 c m . ( v o l u m e 1800 m l . ) with SEPHADEX G - 2 0 0 w a s used for gel filtration of a sample of 50 ml. swine serum. Elution was carried out with 1 M N a C I in 0.1 M tris-HCI buffer p H 8.0.
Sample: 3 ml. of an extract of powdered swine pancreas in 0.05 M acetate buffer pH 5.3 containing 0.005 M calcium acetate. Column size: 1)7 ml. (2 χ 37 cm.ï. Elution: 0.05 M acetate buffer pH 5.3 containing 0.005 M calcium acetate.
What they promise Evidently sE
the new types of SEPHADEX m a k e it possible to fractionate high polymers such as plasma proteins, h o r m o n e s , a n d poly saccharides on preparative scale. Also available: SEPHADEX G - 2 5 , G - 5 0 a n d G - 7 5 .
PHADEX
G - 1 0 0 and G - 2 0 0 cover a field of e n o r m o u s interest, especially for biochemical and medical research. A s well as superseding earlier highly time-consuming analytical methods,
PHARMACIA LEADING IN DEXTRAN CHEMISTRY
For full informa l/on on the use of SEPHADEX in gel filtration, send the coupon with your letterhead. (Inquiries outside the Western Hemisphere should be directed to PHARMACIA, Uppsala, Sweden.)
Elution: 1 M NaCI in 0.1 M tris-HCI buffer pH 8.0.
PHARMACIA FINE CHEMICALS, INC. Department A C 501 Fifth Avenue, New York 17, New York Please send the f o l l o w i n g : G-100 and G-200 I 1 "SEPHADEX": Λ Unique BROCHURE I 1 Substance for Modern Chromatography
•
I I
1 "SEPHADEX in Gel 1 Filtration": Theory and Experimental Technique
! ! I | I
Name
ι
Company
•
Address
I
Circle No. 163 on Readers' Service Card VOt.
3 6 , N O . 4, APRIL 1 9 6 4 ·
12 5 A
