CHOLESTEROL OXIDASE 100 units $35.00 500 units $145.00 Available from Stock C h o l e s t e r o l o x i d a s e (first d e s c r i b e d b y T. C. S t a d t m a n et al*) offers a s e n s i t i v e e n z y m a t i c m e t h o d f o r t h e quantitative determination of cholesterol. A convenient m e t h o d d e v e l o p e d in o u r l a b o r a t o r i e s i n v o l v e s c o u p l i n g to o-dianisidine w i t h horseradish peroxidase. P-L B i o c h e m i c a l s , Inc. is p l e a s e d t o a n n o u n c e t h e i m m e d i a t e availability o f cholesterol oxidase. C o n d e n s e d specifications are given b e l o w : 0732 Prep: Activity:
Affinity Chromatography Media A w i d e variety of ligands are offered in " r e a d y - t o - u s e c o n d i t i o n " , j u s t wash with 2 - 3 volumes of water a n d proceed w i t h y o u r e x p e r i m e n t . Nucleotide Ligands: AMP (3 types), 3', 5'-Cyclic AMP, ADP, ATP, GTP, UTP
CHOLESTEROL OXIDASE, (Nocardia sp.) T y p e 2
Coenzyme Ligands: NAD (2 types), NADP, Coenzyme A (2 types), Cysteamine
LYOPHILIZED P O W D E R . Partially p u r i f i e d f r o m g l y c e r o l - g r o w n cells.
Polynucleotide Ligands: Poly I, Poly U, Double Strand Poly I • Poly C, Oligo (dT)
M i n i m u m 20 units/mg. protein. O n e unit catalyzes t h e f o r m a t i o n o f e n o u g h H2O2 t o p r o d u c e a c h a n g e o f 0.02 A i 4 0 p e r m i n u t e i n a system c o n t a i n i n g h o r s e r a d i s h p e r o x i d a s e a n d o - d i a n i s i d i n e at 3 7 ° C , p H 6.6. A c o p y o f t h e P-L M e t h o d E732-440 is p r o v i d e d w i t h each o r d e r .
Supports: Agarose and Cellulose
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*Stadtman, T. C , Cherkes, A., and Anfinsen, C. B., (1954) I. Biol. Chem. 206,511-523. Telephone (414) 271-0667
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CIRCLE 493 ON READER SERVICE CARD
CIRCLE 494 ON READER SERVICE CARD
P-L Biochemicals, Inc., proudly announces publication of the latest edition of the
Fluorescent 1,N6 -etheno-derivatives* W h e r e : £ represents [1,N6-etheno-]
INTERMEDIARY ! *• METABOLISM CHARTS*
£ Adenosine £ Adenosine 5'-phosphate
£ AMP Structure Diagram NH
£ Adenosine 5'-diphosphate
H.J. Sallach, Ph.D., Author
£ Adenosine 5'-triphosphate
• Thoroughly up-dated a n d revised £ 2'-Deoxyadenosine 5'-triphosphate
• New sections added • Expanded new 4-chart format (each 2 3 " x 3 4 " ) Chart section shown one-half size.
£ A d e n o s i n e 3 ' , 5 ' , c y c l i c p h o s p h a t e {£ C A M P ) £ Coenzyme A
• New spiral b i n d i n g a n d wall hanger • High quality 5-color r e p r o d u c t i o n on heavy-duty paper
£ Acetyl Coenzyme A
' F o r m e r l y pubished by Gilson Medical Electronics Corp.
COg*
. sets of charts @ $3.75 each • Please s e n d . (less 2 5 % for 2 5 sets or more). Enclosed f i n d $_ (Check or money order only; Do not send cash) • Please send additional information. Name
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£ Nicotinamide-Adenine Dinucleotide (NAD) * T h e synthesis, properties a n d applications of these d e r i v a t i v e s h a v e b e e n d e s c r i b e d i n a series o f a r t i c l e s b y D r . N e l s o n J. L e o n a r d a n d associates a t t h e U n i v e r s i t y of I l l i n o i s , U r b a n a (eg. see: B i o c h e m i s t r y 1 1 , 3 4 9 9 (1972). W r i t e o r call f o r f u l l d e t a i l s : (414) 271 - 0 6 6 7 , Telex 26S81
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PLbiochemicals,inc. t P. O. Box 4606, Chicago, Illinois 60680, U.S.A. CIRCLE 496 ON READER SERVICE CARD 230 LG AUGUST 1974
PL bkxdhemicals, inc. 1037NNEST McKINLEY AVENUE. /V1IIW4UKEE. W I S 5 3 2 0 5 CIRCLE 495 ON READER SERVICE CARD LABORATORY
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